Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
J Biosci Bioeng. 2019 Sep;128(3):365-372. doi: 10.1016/j.jbiosc.2019.02.014. Epub 2019 Mar 29.
A solubilized liver-specific extracellular matrix (L-ECM) substratum was obtained by decellularization of porcine liver using Triton X-100 and pepsin treatments. The L-ECM was able to immobilize hepatocyte growth factor at a high efficiency of 87%. L-ECM gelled spontaneously in a physiologically neutral environment. Primary hepatocytes embedded in the L-ECM gel showed a high albumin synthesis activity and ethoxyresorufin-O-deethylase (EROD) activity even at 3 weeks in culture. In addition, the L-ECM gel-embedded hepatocytes implanted subcutaneously into partial hepatectomized rats showed a high survival rate (18%) and formed a large liver tissue-like structure. Their efficiencies of EROD activity and large liver tissue-like structure formation were about twice those of collagen gel-embedded hepatocytes. Based on these results, we clarified the effectiveness of L-ECM gel as a substrate for hepatocyte culture and transplantation.
采用 Triton X-100 和胃蛋白酶处理猪肝脏，可获得一种去细胞化的肝脏特异性细胞外基质（L-ECM）基质。该 L-ECM 能够以 87%的高效率固定肝细胞生长因子。L-ECM 在生理中性环境中可自发凝胶化。包埋在 L-ECM 凝胶中的原代肝细胞在培养 3 周时仍具有很高的白蛋白合成活性和乙氧基荧光素-O-去乙基酶（EROD）活性。此外，将 L-ECM 凝胶包埋的肝细胞植入部分肝切除大鼠的皮下，其存活率（18%）较高，并形成大的肝组织样结构。其 EROD 活性和大肝组织样结构形成的效率大约是胶原凝胶包埋的肝细胞的两倍。基于这些结果，我们阐明了 L-ECM 凝胶作为肝细胞培养和移植的基质的有效性。
