Kragballe K, Pinnamaneni G, Desjarlais L, Duell E A, Voorhees J J
J Invest Dermatol. 1986 Oct;87(4):494-8. doi: 10.1111/1523-1747.ep12455564.
The purpose of the present study was to analyze the metabolism of arachidonic acid (AA) in normal human dermis. After incubating homogenized dermis with exogenous AA, the extracted lipids were isolated by reversed-phase high-performance liquid chromatography. Each chromatographic peak was characterized by its UV absorption spectrum and identified by its coelution with the appropriate authentic standard and by radioimmunoassay of its eluate fraction. Identified compounds were quantitated by integrated optical density. Homogenized human dermis transformed AA into both cyclooxygenase and lipoxygenase products, but predominantly 15-hydroxy-eicosatetraenoic acid (15-HETE). Cultured fibroblasts from normal human dermis also mainly metabolized AA into 15-HETE. To determine whether dermis-derived 15-HETE might modify the AA metabolism of epidermis, normal human epidermis was incubated with dermis. Increasing amounts of dermis resulted in an increasing inhibition of epidermal 12-HETE formation. Similarly, 15-HETE alone induced a dose-dependent decrease of epidermal 12-HETE formation, while the formation of prostaglandin E2 was unchanged. Since 12-HETE possess the ability to elicit skin inflammation and to stimulate epidermal DNA synthesis, 15-HETE formation may be a way whereby dermis regulates important epidermal activities.
本研究的目的是分析正常人真皮中花生四烯酸（AA）的代谢情况。用外源性AA孵育匀浆真皮后，通过反相高效液相色谱法分离提取的脂质。每个色谱峰通过其紫外吸收光谱进行表征，并通过与适当的标准品共洗脱及其洗脱级分的放射免疫测定进行鉴定。鉴定出的化合物通过积分光密度进行定量。匀浆的人真皮将AA转化为环氧化酶和脂氧化酶产物，但主要是15-羟基-二十碳四烯酸（15-HETE）。正常人真皮培养的成纤维细胞也主要将AA代谢为15-HETE。为了确定真皮来源的15-HETE是否可能改变表皮的AA代谢，将正常人表皮与真皮一起孵育。真皮量的增加导致表皮12-HETE形成的抑制增加。同样，单独的15-HETE诱导表皮12-HETE形成呈剂量依赖性降低，而前列腺素E2的形成不变。由于12-HETE具有引发皮肤炎症和刺激表皮DNA合成的能力，15-HETE的形成可能是真皮调节重要表皮活动的一种方式。
