Battista P J, Condon W A
J Reprod Fertil. 1986 Sep;78(1):275-80. doi: 10.1530/jrf.0.0780275.
Incubation of bovine luteal cells with the alternative pathway catecholamines octopamine, synephrine and deoxyadrenaline at concentrations of 10(-6) to 10(-3) M enhanced the production of progesterone (P less than 0.05). Tryamine did not alter basal progesterone production (P greater than 0.05). Addition of noradrenaline and adrenaline at concentrations of 10(-4) to 10(-7) M significantly elevated the production of progesterone (P less than 0.05). The steroidogenic response to noradrenaline and adrenaline was greater than that for octopamine, synephrine and deoxyadrenaline (P less than 0.05). Response to both primary (10(-6) M) and alternative (10(-4) M) pathway catecholamines was inhibited by propranolol (10(-5) M, P less than 0.05) but not phentolamine (10(-5) M, P greater than 0.05). These results demonstrate that octopamine, synephrine and deoxyadrenaline can affect steroidogenesis by bovine luteal cells, and their action is mediated by beta-adrenergic receptors.
将牛黄体细胞与浓度为10(-6)至10(-3) M的替代途径儿茶酚胺章鱼胺、辛弗林和脱氧肾上腺素一起孵育，可提高孕酮的生成量（P<0.05）。酪胺不会改变基础孕酮生成量（P>0.05）。添加浓度为10(-4)至10(-7) M的去甲肾上腺素和肾上腺素可显著提高孕酮的生成量（P<0.05）。对去甲肾上腺素和肾上腺素的类固醇生成反应大于对章鱼胺、辛弗林和脱氧肾上腺素的反应（P<0.05）。普萘洛尔（10(-5) M）可抑制对主要途径（10(-6) M）和替代途径（10(-4) M）儿茶酚胺的反应（P<0.05），但酚妥拉明（10(-5) M）则无此作用（P>0.05）。这些结果表明，章鱼胺、辛弗林和脱氧肾上腺素可影响牛黄体细胞的类固醇生成，且它们的作用是由β-肾上腺素能受体介导的。
