Körholz D, Wahn V, von Kries R, Fabry U, Göbel U
Klin Padiatr. 1986 Jul-Aug;198(4):306-11. doi: 10.1055/s-2008-1033877.
Sera and EDTA-Plasma of patients with severe Haemophilia A were analysed for immune complexes and the hemolytic activity of complement in relation to Factor VIII replacement, in order to confirm or possibly exclude a relationship to allergic reactions. Immune complexes were isolated by PEG precipitation and quantitated. In addition a solid phase ELISA assay was used to detect complement-binding complexes. Total hemolytic complement activity of the classical and the alternate pathway was measured in addition to the C3 splitproduct C3d. The results obtained from 12 patients with severe Haemophilia A showed slightly increased immune complex titers, no changes of the immune complex levels during Factor VIII replacement and no alteration of the complement system following the infusions. One patient developed an allergic reaction without evidence of complement activation.
对重度甲型血友病患者的血清和乙二胺四乙酸血浆进行分析，检测免疫复合物及补体的溶血活性，并与因子Ⅷ替代治疗相关联，以确认或排除与过敏反应的关系。通过聚乙二醇沉淀法分离免疫复合物并进行定量分析。此外，采用固相酶联免疫吸附测定法检测补体结合复合物。除了C3裂解产物C3d外，还测定了经典途径和替代途径的总溶血补体活性。12例重度甲型血友病患者的检测结果显示，免疫复合物滴度略有升高，因子Ⅷ替代治疗期间免疫复合物水平无变化，输注后补体系统无改变。1例患者出现过敏反应，但无补体激活的证据。
