Gemmobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat.

A novel cream-pigmented marine bacterium, designated strain YJ-T1-11, was isolated from a tidal flat at Yeongjong-do, Republic of Korea. Cells were rod-shaped, non-motile, aerobic, Gram-reaction-negative, oxidase-positive and catalase-positive. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain YJ-T1-11 clustered with Gemmobacter fontiphilus JS43 (98.3 %) within the genus Gemmobacter and its closest neighbours were G.emmobacter aquatilis DSM 3857 (98.5 %), Gemmobacter aquaticus A1-9 (98.4 %), Gemmobacterlanyuensis Orc-4 (98.4 %), Gemmobacterfontiphilus JS43 (98.3 %), Gemmobactercaeni DCA-1 (98.2 %), Gemmobacternanjingensis Y12 (97.5 %) and Gemmobactertilapiae Ruye-53 (97.2 %). Average nucleotide identity values between the genome sequences of strain YJ-T1-11 and the related type strains ranged from 77.08 to 90.48 %. The predominant fatty acid of strain YJ-T1-11 was summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c). The major isoprenoid quinone was Q-10 and the DNA G+C content was 65.6 mol%. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and three unidentified lipids. The DNA-DNA relatedness values between strain YJ-T1-11 and the type strains of the 12 phylogenetically related species of the genus Gemmobacter were 23.6-53.7 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain YJ-T1-11 is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter lutimaris sp. nov. is proposed. The type strain is YJ-T1-11 (=KCTC 62715=JCM 32828).