Lim Kahao, Kannan Arvind D, Shobnam Nusrat, Mahmood Maheen, Lee Jaejin, Im Jeongdae
Department of Civil Engineering, Kansas State University, Manhattan, KS 66503, USA.
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa 50011, USA.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4224-4232. doi: 10.1099/ijsem.0.004276.
A Gram-stain-negative, long-rod-shaped and facultative aerobic bacterium, designated HB-1, was isolated from a round hay bale at the Kansas State University Beef Stocker Unit. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB-1 clustered within the genus and its closest relatives were A1-9 (98.0 %), YJ-T1-11 (98.0 %), JS43 (97.8 %), DSM 3857 (97.5 %) and Orc-4 (96.9 %). Additional phylogenomic analysis also indicated that strain HB-1 belongs to the genus . The draft genome of strain HB-1 had a total length of 4.23 Mbp and contained 4071 protein-coding genes. The average nucleotide identity values between the genomes of strain HB-1 and the three most-related type strains ranged from 77.5 to 78.1 %. The DNA G+C content of strain HB-1 was 63.7 mol%. The novel strain grew at 10-37 °C, pH 5-10 and with 0-2 % NaCl. Oxidase and catalase activities were positive. Cells were 0.3-0.4 µm wide, 3.0-7.0 µm long and usually found in pairs or chains of cells. The major respiratory quinone of strain HB-1 was Q-10 (90 %), with a minor amount of Q-9 (10 %). The major fatty acids were C ω7 (54.6 %) and C (18.2 %). On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain HB-1 (=DSM 109828=ATCC TSD-211) is considered to represent a novel species of the genus , for which the name sp. nov. is proposed.
从堪萨斯州立大学肉牛育肥场的一个圆形干草捆中分离出一株革兰氏阴性、长杆状兼性需氧细菌，命名为HB-1。16S rRNA基因序列的系统发育分析结果表明，菌株HB-1聚类在该属内，与其亲缘关系最近的是A1-9（98.0%）、YJ-T1-11（98.0%）、JS43（97.8%）、DSM 3857（97.5%）和Orc-4（96.9%）。进一步的全基因组系统发育分析也表明菌株HB-1属于该属。菌株HB-1的基因组草图全长4.23 Mbp，包含4071个蛋白质编码基因。菌株HB-1与三个亲缘关系最密切的模式菌株的基因组之间的平均核苷酸同一性值在77.5%至78.1%之间。菌株HB-1的DNA G+C含量为63.7 mol%。该新菌株在10-37°C、pH 5-10和0-2% NaCl条件下生长。氧化酶和过氧化氢酶活性为阳性。细胞宽0.3-0.4 µm，长3.0-7.0 µm，通常成对或成链状存在。菌株HB-1的主要呼吸醌是Q-10（90%），少量为Q-9（10%）。主要脂肪酸为C ω7（54.6%）和C（18.2%）。根据表型、系统发育和化学分类学数据，菌株HB-1（=DSM 109828=ATCC TSD-211）被认为代表该属的一个新物种，为此提出新物种名为sp. nov.。
