Lasley Stephen M
Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine Peoria, Peoria, Illinois.
Curr Protoc Toxicol. 2019 Jun;80(1):e72. doi: 10.1002/cptx.72. Epub 2019 Apr 2.
The technique of microdialysis permits the assessment of neurotransmitter activity and the monitoring of other cellular entities in tissue extracellular fluid. The method is widely used for quantifying biogenic amine and amino acid transmitters, peptides, administered drugs, and other molecules in response to various experimental treatments. This article provides an overview of the manner in which the methodology of intracerebral microdialysis is utilized in the field of neurotoxicology to elucidate the actions of environmental agents. The technique is employed in a variety of creative ways to address specific experimental goals involving myriad toxicants. With appropriate consideration of method parameters, investigators have also been able to address mechanistic issues in their studies. These investigations consist of sampling of neurotransmitters in extracellular fluid after various protocols of environmental metal exposure as well as assessments of blood-brain barrier permeability, the detection of reactive oxygen species, and description of the toxicodynamics of environmental agents. The purpose of this examination is not to review the investigational findings, per se, but to highlight the various approaches utilized with this methodology and the experimental questions that have been addressed. © 2019 by John Wiley & Sons, Inc.
微透析技术能够评估神经递质活性，并监测组织细胞外液中的其他细胞成分。该方法广泛用于定量生物胺、氨基酸递质、肽、给药药物以及其他分子，以响应各种实验处理。本文概述了脑内微透析方法在神经毒理学领域用于阐明环境因子作用的方式。该技术以多种创新方式用于实现涉及多种毒物的特定实验目标。在适当考虑方法参数的情况下，研究人员还能够在其研究中解决机制问题。这些研究包括在各种环境金属暴露方案后对细胞外液中的神经递质进行采样，以及评估血脑屏障通透性、检测活性氧物种和描述环境因子的毒代动力学。本次审查的目的并非本身去回顾研究结果，而是强调使用该方法所采用的各种方法以及已解决的实验问题。© 2019 约翰威立国际出版公司
