Stelma Gerard N, Crawford Ronald G, Spaulding Procter L, Twedt Robert M
Division of Microbiology, Food and Drug Administration, 1090 Tusculum Avenue, Cincinnati, Ohio 45226.
J Food Prot. 1985 Mar;48(3):232-233. doi: 10.4315/0362-028X-48.3.232.
Thirteen Clostridium perfringens isolates classified as nonenterotoxigenic by radioimmunoassay (RIA) were tested for biological activity in rabbit ileal loops to determine whether these organisms produced enterotoxins serologically unrelated to the classical C. perfringens enterotoxin. None of these strains was active in the ileal loop assays. The large number of RIA-negative isolates obtained from food-poisoning outbreaks is more likely due to the failure to isolate causative strains rather than to the existence of novel enterotoxins.
通过放射免疫测定法（RIA）分类为非产肠毒素型的13株产气荚膜梭菌分离株，在兔回肠袢中进行了生物活性测试，以确定这些菌株是否产生与经典产气荚膜梭菌肠毒素血清学无关的肠毒素。这些菌株在回肠袢试验中均无活性。从食物中毒暴发中获得的大量RIA阴性分离株，更可能是由于未能分离出致病菌株，而非存在新型肠毒素。
