Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252; Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252, Korea.
Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252, Korea.
BMB Rep. 2019 Nov;52(11):635-640. doi: 10.5483/BMBRep.2019.52.11.018.
CpG-DNA triggers the proliferation and differentiation of B cells which results in the increased production of antibodies. The presence of bacteria-reactive IgM in normal serum was reported; however, the relevance of CpG-DNA with the production of bacteria-reactive IgM has not been investigated. Here, we proved the function of CpG-DNA for the production of bacteria-reactive IgM. CpG-DNA administration led to increased production of bacteria-reactive IgM both in the peritoneal fluid and serum through TLR9 signaling pathway. When we stimulated B cells with CpG-DNA, production of bacteria-reactive IgM was reproduced in vitro. We established a bacteria-reactive monoclonal IgM antibody using CpG-DNA stimulated-peritoneal B cells. The monoclonal IgM antibody enhanced the phagocytic activity of RAW 264.7 cells against S. aureus MW2 infection. Therefore, we suggest that CpG-DNA enhances the antibacterial activity of the immune system by triggering the production of bacteria-reactive IgM. We also suggest the possible application of the antibodies for the treatment of antibiotics-resistant bacterial infections. [BMB Reports 2019; 52(11): 635-640].
CpG-DNA 可触发 B 细胞的增殖和分化，导致抗体产生增加。有报道称正常血清中存在针对细菌的 IgM，但 CpG-DNA 与产生针对细菌的 IgM 的相关性尚未得到研究。在这里，我们证明了 CpG-DNA 对产生针对细菌的 IgM 的功能。CpG-DNA 通过 TLR9 信号通路给药导致腹腔液和血清中针对细菌的 IgM 产生增加。当我们用 CpG-DNA 刺激 B 细胞时，体外重现了针对细菌的 IgM 的产生。我们使用 CpG-DNA 刺激的腹腔 B 细胞建立了针对细菌的单克隆 IgM 抗体。该单克隆 IgM 抗体增强了 RAW 264.7 细胞对 MW2 金黄色葡萄球菌感染的吞噬活性。因此，我们认为 CpG-DNA 通过触发针对细菌的 IgM 的产生来增强免疫系统的抗菌活性。我们还建议针对细菌的抗体可用于治疗对抗生素耐药的细菌感染。[BMB 报告 2019；52(11)：635-640]。
