CpG-DNA 的抗菌作用涉及补体系统的增强。
Anti-Bacterial Effect of CpG-DNA Involves Enhancement of the Complement Systems.
机构信息
Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Korea.
Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252, Korea.
出版信息
Int J Mol Sci. 2019 Jul 10;20(14):3397. doi: 10.3390/ijms20143397.
CpG-DNA activates the host immune system to resist bacterial infections. In this study, we examined the protective effect of CpG-DNA in mice against () K1 infection. Administration of CpG-DNA increased the survival of mice after K1 infection, which reduces the numbers of bacteria in the organs. Pre-injection of mice with CpG-DNA before K1 infection increased the levels of the complement C3 but not C3a and C3b. The survival of the mice after K1 infection was significantly decreased when the mice were pre-injected with the cobra venom factor (CVF) removing the complement compared to the non-CVF-treated mice group. It suggests that the complement has protective roles against K1 infection. In addition, the survival of complement-depleted mice was increased by CpG-DNA pre-administration before K1 infection. Therefore, we suggest that CpG-DNA enhances the anti-bacterial activity of the immune system by augmenting the levels of complement systems after K1 infection and triggering other factors as well. Further studies are required to investigate the functional roles of the CpG-DNA-induced complement regulation and other factors against urgent bacterial infection.
CpG-DNA 可激活宿主免疫系统以抵抗细菌感染。在本研究中，我们研究了 CpG-DNA 对（）K1 感染小鼠的保护作用。CpG-DNA 的给药增加了 K1 感染后小鼠的存活率，减少了器官中的细菌数量。在 K1 感染前预先注射 CpG-DNA 可增加补体 C3 的水平，但不增加 C3a 和 C3b 的水平。与未用 cobra venom factor (CVF) 处理的小鼠组相比，用 CVF 预处理以去除补体的小鼠在 K1 感染后的存活率显著降低。这表明补体对 K1 感染具有保护作用。此外，CpG-DNA 预先给药可增加补体耗竭小鼠的存活率。因此，我们认为 CpG-DNA 通过在 K1 感染后增加补体系统的水平并触发其他因素来增强免疫系统的抗细菌活性。需要进一步研究以研究 CpG-DNA 诱导的补体调节和其他因素对紧急细菌感染的功能作用。
Zotero 插件