Department of Biochemistry, University of Illinois, 600 S. Mathews Street, Urbana, IL 61801, United States.
Department of Biochemistry, University of Illinois, 600 S. Mathews Street, Urbana, IL 61801, United States.
Chem Phys Lipids. 2019 Jul;221:114-119. doi: 10.1016/j.chemphyslip.2019.03.007. Epub 2019 Mar 30.
Styrene-maleic acid (SMA) copolymers can extract membrane proteins from native membranes along with lipids as nanodiscs. Preparation with SMA is fast, cost-effective, and captures the native protein-lipid interactions. On the other hand, cryo-EM has become increasingly successful and efficient for structural determinations of membrane proteins, with biochemical sample preparation often the bottleneck. Three recent cryo-EM studies on the efflux transporter AcrB and the alternative complex III: cyt c oxidase supercomplex have demonstrated the potential of SMA nanodisc samples to yield high-resolution structure information of membrane proteins.
苯乙烯-马来酸共聚物可以将膜蛋白和脂质一起从天然膜中提取出来，形成纳米盘。用 SMA 制备是快速、经济高效的，并且可以捕获天然的蛋白质-脂质相互作用。另一方面，冷冻电镜对于膜蛋白的结构测定已经越来越成功和高效，而生化样品制备通常是瓶颈。最近有三项关于外排转运蛋白 AcrB 和替代复合物 III：细胞色素 c 氧化酶超级复合物的冷冻电镜研究表明，SMA 纳米盘样品有潜力提供膜蛋白的高分辨率结构信息。
