形成 pH 耐受的单分散聚合物-脂质纳米盘。
Formation of pH-Resistant Monodispersed Polymer-Lipid Nanodiscs.
机构信息
Biophysics Program and Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055, USA.
出版信息
Angew Chem Int Ed Engl. 2018 Jan 26;57(5):1342-1345. doi: 10.1002/anie.201712017. Epub 2018 Jan 8.
Abstract
Polymer lipid nanodiscs are an invaluable system for structural and functional studies of membrane proteins in their near-native environment. Despite the recent advances in the development and usage of polymer lipid nanodisc systems, lack of control over size and poor tolerance to pH and divalent metal ions are major limitations for further applications. A facile modification of a low-molecular-weight styrene maleic acid copolymer is demonstrated to form monodispersed lipid bilayer nanodiscs that show ultra-stability towards divalent metal ion concentration over a pH range of 2.5 to 10. The macro-nanodiscs (>20 nm diameter) show magnetic alignment properties that can be exploited for high-resolution structural studies of membrane proteins and amyloid proteins using solid-state NMR techniques. The new polymer, SMA-QA, nanodisc is a robust membrane mimetic tool that offers significant advantages over currently reported nanodisc systems.
摘要
聚合物脂质纳米盘是一种非常有价值的系统，可用于在近天然环境下研究膜蛋白的结构和功能。尽管聚合物脂质纳米盘系统在开发和使用方面取得了最近的进展，但对尺寸缺乏控制以及对 pH 值和二价金属离子的耐受性差仍然是进一步应用的主要限制。本文证明，通过对低分子量苯乙烯马来酸共聚物进行简单修饰，可以形成单分散脂质双层纳米盘，在 pH 值为 2.5 到 10 的范围内对二价金属离子浓度具有超稳定性。大纳米盘（>20nm 直径）显示出磁对准性质，可用于使用固态 NMR 技术对膜蛋白和淀粉样蛋白进行高分辨率结构研究。新型聚合物 SMA-QA 纳米盘是一种稳健的膜模拟工具，相对于目前报道的纳米盘系统具有显著优势。
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