Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, 08903, USA.
Department of Medicine, Rutgers-Robert Wood Johnson Medical School, New Brunswick, NJ, 08901, USA.
Anal Biochem. 2019 Jun 15;575:40-43. doi: 10.1016/j.ab.2019.03.020. Epub 2019 Mar 30.
Glucose and glycerol are important circulating metabolites. Due to poor ionization and/or ion suppression, the liquid chromatography-mass spectrometry (LC-MS) detection of glucose and glycerol presents challenges. Here, we propose an efficient LC-MS method of quantitative glucose and glycerol detection via enzymatic derivatization to glucose-6-phosphate and sn-glycerol-3-phosphate, respectively. This derivatization protocol can be used to measure the concentrations of glucose production in a plethora of sample types for metabolic analysis and is compatible with the general metabolomics workflow. This novel approach allows us to quantitatively study glucose and glycerol metabolism using stable isotope tracers in vivo.
葡萄糖和甘油是重要的循环代谢物。由于电离不良和/或离子抑制，液相色谱-质谱（LC-MS）检测葡萄糖和甘油具有一定挑战性。本文中，我们提出了一种高效的 LC-MS 方法，通过酶衍生化分别将葡萄糖和甘油定量转化为葡萄糖-6-磷酸和 sn-甘油-3-磷酸，实现对葡萄糖和甘油的检测。该衍生化方案可用于代谢分析中多种样本类型的葡萄糖生成浓度测量，且与常规代谢组学工作流程兼容。该新方法使我们能够使用稳定同位素示踪剂在体内对葡萄糖和甘油代谢进行定量研究。
