Mammalian Embryo and Stem Cell Group, University of Cambridge, Department of Physiology, Development and Neuroscience, Downing Street, Cambridge, CB2 3DY, UK.
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Bailrigg, Furness Building, Lancaster LA1 4YG, UK.
Dev Biol. 2019 Jun 15;450(2):132-140. doi: 10.1016/j.ydbio.2019.03.016. Epub 2019 Mar 30.
Migration of Anterior Visceral Endoderm (AVE) is a critical symmetry breaking event in the early post-implantation embryo development and is essential for establishing the correct body plan. Despite much effort, cellular and molecular events influencing AVE migration are only partially understood. Here, using time-lapse live imaging of mouse embryos, we demonstrate that cell division in the embryonic visceral endoderm is coordinated with AVE migration. Moreover, we demonstrate that temporal inhibition of FGF signalling during the pre-implantation specification of embryonic visceral endoderm perturbs cell cycle progression, thus affecting AVE migration. These findings demonstrate that coordinated cell cycle progression during the implantation stages of development is important for post-implantation morphogenesis in the mouse embryo.
原肠内胚层（AVE）的迁移是早期胚胎植入后发育中一个关键的对称破缺事件，对于建立正确的身体形态至关重要。尽管已经做了很多努力，但影响 AVE 迁移的细胞和分子事件仍未被完全理解。在这里，我们通过对小鼠胚胎的延时实时成像，证明了胚胎内脏内胚层的细胞分裂与 AVE 迁移相协调。此外，我们还证明了在胚胎内脏内胚层的着床前特化过程中，短暂抑制 FGF 信号会干扰细胞周期进程，从而影响 AVE 迁移。这些发现表明，在胚胎植入阶段协调的细胞周期进程对于小鼠胚胎的植入后形态发生是重要的。
