McCarthy J H
Exp Hematol. 1978 Oct;6(9):709-17.
Intact sheep red cells potentiated mouse B lymphocyte colony growth in agar but red cell membranes or lysates exhibited no comparable ability. For maximum colony formation red cells had to be present throughout the entire culture period. Red cells added to cultures late in the culture period did not potentiate growth. Intimate contact between colony-forming cells and red cells was not essential for potentiation to occur. Red cell lysates inhibited normal B lymphocyte colony formation in cultures containing intact red cells, but did not inhibit colony formation by cells of the B lymphoid leukemia ABE-8. This differential effect may provide a means of differentiating normal from neoplastic colony-forming B lymphocytes in the mouse. Eight different tumors were also examined. Intact red cells potentiated colony formation by all of them. Lysed red cells did not potentiate the growth of any of the tumor lines. The mastocytoma P815 was the only tumor whose colony formation was inhibited by the addition of intact and lysed red cells.
完整的绵羊红细胞可增强小鼠B淋巴细胞在琼脂中的集落生长，但红细胞膜或裂解物则没有类似的能力。为了实现最大程度的集落形成，在整个培养期间都必须存在红细胞。在培养后期添加到培养物中的红细胞并不能增强生长。集落形成细胞与红细胞之间的密切接触对于增强作用的发生并非必不可少。红细胞裂解物在含有完整红细胞的培养物中抑制正常B淋巴细胞集落形成，但不抑制B淋巴样白血病ABE-8细胞的集落形成。这种差异效应可能提供一种区分小鼠中正常与肿瘤性集落形成B淋巴细胞的方法。还检查了八种不同的肿瘤。完整的红细胞增强了所有肿瘤的集落形成。裂解的红细胞不能增强任何肿瘤系的生长。肥大细胞瘤P815是唯一一种添加完整和裂解红细胞后其集落形成受到抑制的肿瘤。
