Claesson M H, Metcalf D
J Immunol. 1977 Apr;118(4):1208-12.
Mercatoethanol-induced B lymphocyte cloning in semi-solid agar has been used to study lymphocyte colony formation by cells from the SJL/J mouse thymus. From the 3rd month of life, the SJL/J mouse thymus. From the 3rd month of life, the SJL thymus develops an increasing frequency of cells forming B lymphocyte colonies in agar. The peak frequency in 6- to 12-month-old mice was one colony per 1000 to 2000 cultured thymus cells. In contrast, 10 to 100 times lower frequencies were found in the thymus of five other inbred mouse strains. The rise in B lymphocyte colony-forming cells correlated well with the age-related rise in Ig-positive cells and approximately 50% of the colony cells reacted with anti-micron-serum indicating the B lymphocyte nature of the colony cells. Colony-forming cells from the thymus showed higher sensitivity than colony-forming spleen cells to cortisol and irradiation. Cell transfer experiments and thymus grafting suggested that the increased frequency of colony-forming cells in the thymus is caused by development of special thymus-seeking B lymphocytes in ageing SJL/J mice. Finally, B lymphocyte colony-forming cells were found to be more frequent in the thymus, spleen, and lymph nodes from healthy aged mice than in lymphoid organs from mice with spontaneous reticulum cell tumors.
巯基乙醇诱导的半固体琼脂中B淋巴细胞克隆已被用于研究SJL/J小鼠胸腺细胞的淋巴细胞集落形成。从出生后第3个月开始，SJL/J小鼠胸腺。从出生后第3个月开始，SJL胸腺中在琼脂中形成B淋巴细胞集落的细胞频率不断增加。6至12月龄小鼠的峰值频率为每1000至2000个培养的胸腺细胞中有一个集落。相比之下，在其他五个近交系小鼠品系的胸腺中发现的频率低10至100倍。B淋巴细胞集落形成细胞的增加与Ig阳性细胞随年龄的增加密切相关，约50%的集落细胞与抗μ链血清反应，表明集落细胞的B淋巴细胞性质。胸腺中的集落形成细胞比脾中的集落形成细胞对皮质醇和辐射更敏感。细胞转移实验和胸腺移植表明，胸腺中集落形成细胞频率的增加是由衰老的SJL/J小鼠中特殊的归巢B淋巴细胞的发育引起的。最后，发现健康老年小鼠的胸腺、脾脏和淋巴结中的B淋巴细胞集落形成细胞比患有自发性网状细胞瘤的小鼠的淋巴器官中的更频繁。
