Vincent J E, Zijlstra F J, vd Broek A M, Gezel T E
Prostaglandins. 1986 Jul;32(1):132-6. doi: 10.1016/0090-6980(86)90155-3.
The effect of adrenalectomy on the formation of cyclo-oxygenase and lipoxygenase products by activated peritoneal rat macrophages was determined and compared with that of the spleen. After isolation, the cells and tissues were incubated with [1-14C] arachidonic acid and the Ca-ionophore A23187 and the metabolites isolated by HPLC chromatography. The main components formed in the macrophages of the controls are 6-keto-PGF1 alpha, TxB2 and 12-HETE. One peak represents 5, 12 di HETE. Smaller amounts of PGF2 alpha, PGE2, PGD2, LTB4 and 15-HETE are also present. After adrenalectomy, a considerable increase occurs in the amounts of LTB4, 15-HETE and 12-HETE. The increase in the PG is smaller. The compounds formed from endogenous arachidonic acid are also determined. In the cells of the controls, the formation of LTB4 is considerably increased after adrenalectomy. In the spleen, PGD2 and 12-HETE are decreased after adrenalectomy. The effect of the macrophages is most probably related to a diminished amount or inactivation of lipocortin, a glucocorticosteroid induced peptide with PlA2 inhibitory activity in adrenalectomized animals. In the decrease in formation in the spleen, the absence of the permissive effect of glucocorticosteroids on the hormone-induced lipolysis may play a role.
测定了肾上腺切除术对活化的大鼠腹膜巨噬细胞中环氧化酶和脂氧合酶产物形成的影响，并与脾脏的影响进行了比较。分离后，将细胞和组织与[1-¹⁴C]花生四烯酸和钙离子载体A23187一起孵育，并用高效液相色谱法分离代谢产物。对照组巨噬细胞中形成的主要成分是6-酮-PGF1α、TXB2和12-HETE。一个峰代表5,12-二HETE。还存在少量的PGF2α、PGE2、PGD2、LTB4和15-HETE。肾上腺切除术后，LTB4、15-HETE和12-HETE的量显著增加。PG的增加较小。还测定了由内源性花生四烯酸形成的化合物。在对照组细胞中，肾上腺切除术后LTB4的形成显著增加。在脾脏中，肾上腺切除术后PGD2和12-HETE减少。巨噬细胞的这种作用很可能与肾上腺切除动物中脂皮质素（一种具有磷脂酶A2抑制活性的糖皮质激素诱导肽）的量减少或失活有关。在脾脏形成减少方面，糖皮质激素对激素诱导的脂肪分解的允许作用的缺失可能起作用。
