Bielińska A, Hulanicka D
Acta Biochim Pol. 1986;33(2):133-7.
It was found by Northern-type hybridization that the amount of RNA, the transcription of which begins at the cysB regulatory gene promoter, is significantly reduced after cysB gene introduction into Escherichia coli on multicopy plasmid. This result indicates that cysB protein inhibits the transcription of its own gene. O-acetyl-L-serine, an internal inducer of E. coli and Salmonella typhimurin cysteine regulons, has no effect on cysB gene expression.
通过Northern杂交发现，转录起始于cysB调控基因启动子的RNA量，在多拷贝质粒上向大肠杆菌导入cysB基因后显著减少。该结果表明，cysB蛋白抑制其自身基因的转录。O-乙酰-L-丝氨酸，一种大肠杆菌和鼠伤寒沙门氏菌半胱氨酸调节子的内源诱导剂，对cysB基因表达没有影响。
