Pinter E, Reece E A, Leranth C Z, Garcia-Segura M, Hobbins J C, Mahoney M J, Naftolin F
Am J Obstet Gynecol. 1986 Oct;155(4):691-702. doi: 10.1016/s0002-9378(86)80001-1.
Light microscopic, electron microscopic, and morphometric studies were performed on rat conceptuses cultured between day 10 and day 12 in normal, hyperglycemic, arachidonic acid-supplemented normal, and arachidonic acid-supplemented hyperglycemic rat serum. The results were compared with those of 12-day-old conceptuses grown in utero. No major differences were observed between in vivo and in vitro control conceptuses. Arachidonic acid supplementation of control culture medium resulted in an improvement of conceptus development. Addition of 20 micrograms/ml of arachidonic acid to an otherwise teratogenic hyperglycemic serum medium (950 mg/dl of D-glucose) prevented the malformations induced by hyperglycemic conditions: open neural tube, advanced neuropil formation in the neuroepithelium, significant reduction of rough endoplasmic reticulum, decreased size and number of lipid droplets, and increased number of lysosome-like structures in the visceral endodermal yolk sac cells.
对在正常、高血糖、添加花生四烯酸的正常以及添加花生四烯酸的高血糖大鼠血清中于第10天至第12天培养的大鼠胚胎进行了光学显微镜、电子显微镜和形态计量学研究。将结果与在子宫内生长的12日龄胚胎的结果进行了比较。在体内和体外对照胚胎之间未观察到重大差异。在对照培养基中添加花生四烯酸可改善胚胎发育。向原本具有致畸性的高血糖血清培养基（950mg/dl D-葡萄糖）中添加20微克/毫升花生四烯酸可预防高血糖条件诱导的畸形：开放性神经管、神经上皮中神经毡形成提前、粗面内质网显著减少、脂滴大小和数量减少以及内脏内胚层卵黄囊细胞中溶酶体样结构数量增加。
