MicroRNA-663 通过抑制 TUSC2 促进卵巢癌细胞的生长、迁移和侵袭。

MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2.

机构信息

Obstetrics and Gynecology, Xuzhou Central Hospital, Xuzhou, Jiangsu, China.

Department of Integrated Traditional Chinese and Western Medicine, The Third Affiliated Hospital of Kunming Medical University (The Tumor Hospital of Yunnan Province), Kunming, Yunnan, China.

出版信息

Biol Res. 2019 Apr 3;52(1):18. doi: 10.1186/s40659-019-0219-6.

BACKGROUND

MicroRNAs (miRNAs) have emerged as the critical modulators of the tumorigenesis and tumor progression.

METHODS

The levels of miR-663 in ovarian cancer cell lines and clinical tissues were detected using qRT-PCR assays. The Transwell invasion and wound healing assay were conducted to assess the roles of miR-663 in the migration and invasion of ovarian cancer cell in vitro. Rescue assays were carried out to confirm the contribution of tumor suppressor candidate 2 (TUSC2) in the aggressiveness of cancer cell which was regulated by miR-663.

RESULTS

The levels of miR-663 were up-regulated in ovarian cancer tissues in comparison with the corresponding normal tissues. Up-regulation of miR-663 increased the proliferation, colony formation, migration and invasion of ovarian cancer SKOV3 cell. Additional, over-expression of miR-663 increased the tumor growth of SKOV3 in xenograft model. Bioinformatics analysis and luciferase reporter assay identified that miR-663 decreased the level of TUSC2 via binding to the 3'-UTR of TUSC2 gene. Finally, the expression of TUSC2 was inversely associated with the level of miR-663 in ovarian carcinoma tissue and over-expression of TUSC2 inhibited the migration and invasion abilities of SKOV3 that was promoted by miR-663.

CONCLUSION

Altogether, these results indicate that miR-663 acts as a potential tumor-promoting miRNA through targeting TUSC2 in ovarian cancer.

背景

MicroRNAs (miRNAs) 已成为肿瘤发生和肿瘤进展的关键调节因子。

方法

使用 qRT-PCR 检测卵巢癌细胞系和临床组织中 miR-663 的水平。通过 Transwell 侵袭和划痕愈合实验评估 miR-663 在卵巢癌细胞体外迁移和侵袭中的作用。进行挽救实验以确认肿瘤抑制候选物 2 (TUSC2) 在 miR-663 调节的癌细胞侵袭性中的作用。

结果

与相应的正常组织相比，卵巢癌组织中 miR-663 的水平上调。miR-663 的上调增加了卵巢癌 SKOV3 细胞的增殖、集落形成、迁移和侵袭。此外，过表达 miR-663 增加了 SKOV3 在异种移植模型中的肿瘤生长。生物信息学分析和荧光素酶报告基因实验表明，miR-663 通过结合 TUSC2 基因的 3'UTR 降低了 TUSC2 的水平。最后，TUSC2 的表达与卵巢癌组织中 miR-663 的水平呈负相关，过表达 TUSC2 抑制了 miR-663 促进的 SKOV3 的迁移和侵袭能力。