Gouveia Joao D, Lian Jie, Steinert Georg, Smidt Hauke, Sipkema Detmer, Wijffels Rene H, Barbosa Maria J
Bioprocess Engineering, Wageningen University & Research, Wageningen, The Netherlands.
Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.
PeerJ. 2019 Mar 27;7:e6610. doi: 10.7717/peerj.6610. eCollection 2019.
(Chlorophyta) is a green microalga known for producing hydrocarbons and exopolysaccharides (EPS). Improving the biomass productivity of and hence, the productivity of the hydrocarbons and of the EPS, will make more attractive for industries. Microalgae usually cohabit with bacteria which leads to the formation of species-specific communities with environmental and biological advantages. Bacteria have been found and identified with a few strains, but little is known about the bacterial community across the different strains. A better knowledge of the bacterial community of will help to optimize the biomass productivity, hydrocarbons, and EPS accumulation. To better understand the bacterial community diversity of , we screened 12 strains from culture collections. Using 16S rRNA gene analysis by MiSeq we described the bacterial diversity across 12 strains and identified possible shared communities. We found three bacterial families common to all strains: , , and . Additionally, the results also suggest that each strain has its own specific bacteria that may be the result of long-term isolated culture.
绿藻门是一种绿色微藻，以产生碳氢化合物和胞外多糖（EPS）而闻名。提高绿藻门的生物质生产力，进而提高碳氢化合物和EPS的生产力，将使绿藻门对工业更具吸引力。微藻通常与细菌共生，这导致形成具有环境和生物学优势的物种特异性群落。已发现并鉴定了一些绿藻门菌株中的细菌，但对于不同菌株间的细菌群落了解甚少。更好地了解绿藻门的细菌群落将有助于优化生物质生产力、碳氢化合物和EPS积累。为了更好地理解绿藻门的细菌群落多样性，我们从培养物保藏中心筛选了12个菌株。通过MiSeq对16S rRNA基因进行分析，我们描述了12个绿藻门菌株的细菌多样性，并鉴定了可能的共有群落。我们发现所有菌株共有三个细菌科：、和。此外，结果还表明每个菌株都有其自身特定的细菌，这可能是长期分离培养的结果。
