Liu Jinyan, Liu Yingjie, Donkersley Philip, Dong Yonghao, Chen Xi, Zang Yun, Xu Pengjun, Ren Guangwei
Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, People's Republic of China.
Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, UK.
Arch Virol. 2019 Jun;164(6):1567-1573. doi: 10.1007/s00705-019-04231-y. Epub 2019 Apr 3.
Potato virus Y (PVY) is a common pathogen affecting agricultural production worldwide and is mainly transmitted by Myzus persicae in a non-persistent manner. Insect-borne plant viruses can modify the abundance, performance, and behavior of their vectors by altering host plant features; however, most studies have overlooked the fact that the dynamic progression of virus infection in plants can have variable effects on their vectors. We addressed this point in the present study by dividing the PVY infection process in tobacco into three stages (early state, steady state and late state); delineated by viral copy number. We then compared the differential effects of PVY-infected tobacco (Nicotiana tabacum) plants on the host selection and feeding behavior of M. persicae. We used Y-shaped olfactory apparatus and electrical penetration graph (EPG) methods to evaluate host selection and feeding behavior, respectively. Interestingly, we found that PVY-infected plants at the steady state attracted more aphids than healthy plants, whereas no differences were observed for those at the early and late states. In terms of feeding behavior, intracellular punctures (closely related to PVY acquisition and transmission) were more abundant on PVY-infected tobacco plants at the early and steady states of infection than in uninfected plants. These results indicate that PVY-infected host plants can alter the host selection and feeding behavior of aphids in a stage-dependent manner, which is an important consideration when studying the interactions among host plants, viruses, and insect vectors.
马铃薯Y病毒（PVY）是一种影响全球农业生产的常见病原体，主要由桃蚜以非持久性方式传播。昆虫传播的植物病毒可通过改变寄主植物特征来改变其传播介体的数量、性能和行为；然而，大多数研究忽略了植物中病毒感染的动态进程对其传播介体可能产生不同影响这一事实。在本研究中，我们通过将烟草中PVY的感染过程分为三个阶段（早期、稳定期和晚期）来解决这一问题，这三个阶段由病毒拷贝数来界定。然后，我们比较了感染PVY的烟草植株对桃蚜寄主选择和取食行为的不同影响。我们分别使用Y形嗅觉仪和刺探电位图谱（EPG）方法来评估寄主选择和取食行为。有趣的是，我们发现处于稳定期的感染PVY的植株比健康植株吸引更多蚜虫，而处于早期和晚期的植株之间未观察到差异。在取食行为方面，在感染早期和稳定期的感染PVY的烟草植株上，与PVY获取和传播密切相关的细胞内穿刺比未感染植株上更为频繁。这些结果表明，感染PVY的寄主植物可依阶段改变蚜虫的寄主选择和取食行为，这是研究寄主植物、病毒和昆虫传播介体之间相互作用时的一个重要考量因素。
