Fingu-Mabola Junior Corneille, Bawin Thomas, Francis Frédéric
Entomologie Fonctionnelle et Évolutive, Terra, Gembloux Agro-Bio Tech, Liège-Université, Passage des Déportés 2, 5030 Gembloux, Belgium.
Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Framstredet 39, 2019 Tromsø, Norway.
Insects. 2021 Jan 21;12(2):89. doi: 10.3390/insects12020089.
Aphids are major crop pests that transmit more than half of all insect-vectored plant viruses responsible for high yield losses worldwide. Entomopathogenic fungi (EPF) are biological control agents mainly used by foliar application to control herbivores, including sap-sucking pests such as aphids. Their ability to colonize plant tissues and to interact with diverse plant pathogenic microorganisms have been reported. In our study, we evaluated the effectiveness of ((Balsamo-Crivelli) Vuillemin) directly applied by contact or/and indirectly via endophytism in tobacco plants ( L.) against the virus vector (Sulzer) carrying the Potato leafroll virus (PLRV) or not. We found that both contact treatment and endophytic colonization of leaves significantly increased aphid mortality and decreased the fecundity rate when compared to control plants. In addition, on fungal-colonized leaves, viruliferous aphids were more negatively impacted than virus-free ones and nymph mortality was significantly higher than on fungal-free plants. Furthermore, we assessed PLRV transmission by on tobacco plants inoculated with either or ((Driver and Milner) JF Bischoff, Rehner, and Humber) as source or/and recipient plants. was found to acquire and transmit PLRV regardless of the treatment. Nevertheless, the infection rate of endophytically colonized plants was lower at a seven-day incubation period and had increased to almost 100% after fifteen days. These results suggest that is effective against aphids, both by contact and via endophytism, and both and delayed PLRV infection in tobacco.
蚜虫是主要的农作物害虫，传播全球半数以上由昆虫传播的植物病毒，导致农作物产量大幅损失。昆虫病原真菌（EPF）是主要通过叶面喷施来防治食草动物的生物防治剂，包括蚜虫等吸食汁液的害虫。已有报道称其能够定殖于植物组织并与多种植物病原微生物相互作用。在我们的研究中，我们评估了直接通过接触或/和通过内生菌作用间接应用于烟草植株（Nicotiana tabacum L.）的球孢白僵菌（Beauveria bassiana (Balsamo-Crivelli) Vuillemin）对携带或不携带马铃薯卷叶病毒（PLRV）的病毒载体桃蚜（Myzus persicae (Sulzer)）的防治效果。我们发现，与对照植株相比，接触处理和叶片的内生菌定殖均显著提高了蚜虫死亡率并降低了繁殖率。此外，在真菌定殖的叶片上，带毒蚜虫受到的负面影响比无毒蚜虫更大，若虫死亡率显著高于无真菌的植株。此外，我们评估了在接种球孢白僵菌或布氏白僵菌（Beauveria brongniartii (Driver and Milner) JF Bischoff, Rehner, and Humber）作为源植物或/和受体植物的烟草植株上，桃蚜对PLRV的传播情况。无论处理如何，均发现桃蚜能够获取并传播PLRV。然而，内生菌定殖植株在7天潜伏期的感染率较低，15天后几乎增加到100%。这些结果表明，球孢白僵菌通过接触和内生菌作用对蚜虫均有效，球孢白僵菌和布氏白僵菌均可延缓烟草中PLRV的感染。
