核黄素的生物合成。枯草芽孢杆菌的重核黄素合酶催化形成6,7-二甲基-8-核糖基 lumazine。

Biosynthesis of riboflavin. Enzymatic formation of 6,7-dimethyl-8-ribityllumazine by heavy riboflavin synthase from Bacillus subtilis.

作者信息

Neuberger G, Bacher A

出版信息

Biochem Biophys Res Commun. 1986 Sep 30;139(3):1111-6. doi: 10.1016/s0006-291x(86)80292-3.

Abstract

The beta subunits of heavy riboflavin synthase catalyze the formation of 6,7-dimethyl-8-ribityllumazine from 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione and a carbohydrate phosphate, Compound X. 5-Amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate is not a substrate for the enzyme, although it is an established intermediate in the biosynthesis of riboflavin. It follows that this pyrimidine phosphate must be dephosphorylated prior to the formation of 6,7-dimethyl-8-ribityllumazine.

摘要

重核黄素合酶的β亚基催化由5-氨基-6-核糖基氨基-2,4(1H,3H)-嘧啶二酮和一种碳水化合物磷酸酯（化合物X）形成6,7-二甲基-8-核糖基卢马嗪。5-氨基-6-核糖基氨基-2,4(1H,3H)-嘧啶二酮5'-磷酸不是该酶的底物，尽管它是核黄素生物合成中已确定的中间体。由此可见，这种嘧啶磷酸酯在形成6,7-二甲基-8-核糖基卢马嗪之前必须去磷酸化。