Liu Xia, She Chang, Zhong Huan, Liu Tao, Cao Jia-Nan, Zhang Chi, Liu Mi, Chang Xiao-Rong
College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208, China.
Changsha Hopital of TCM (Changsha 8th Hospital), Changsha 410002.
Zhen Ci Yan Jiu. 2019 Feb 25;44(2):113-9. doi: 10.13702/j.1000-0607.180149.
To observe the effect of moxibustion at "Zusanli" (ST36) on metabolites of gastric tissue in chronic atrophic gastritis (CAG) rats, so as to explore the correlation between Yangming Meridian and stomach based on metabolomics.
Fifty-six SD rats were randomly divided into normal, normal ＋ moxibustion, model and model ＋ moxibustion groups (＝14 rats/group). The CAG model was established by gavage of N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) solution and irregular diet for 12 weeks. Moxibustion was applied to ST36 and "Zhongwan" (CV12) for 15 min, once daily for two weeks in the normal＋ moxibustion group and model＋moxibustion group. The histopathological changes of the gastric tissue were observed by H．E. staining. H nuclear magnetic resonance (H NMR) spectroscopy was used to detect gastric tissue metabolites and the data were analyzed using the Chenomx NMR Suite software.
After modeling, the regional gastric tissue showed pale thinner gastric wall, and reduction of regional glands with necrosis and disordered arrangement, epithelial cell exfoliation, lymphocyte infiltration as well as scattered punctate hemorrhage, which was evidently milder in the model ＋ moxibustion group. The metabolites of lactic acid, glutathione, N-acetylaspartate, uridine diphosphate glucose and muscle sugar in the gastric tissue in the model group were increased compared with those in the normal group (<0.05), and the metabolites of leucine, betaine, phosphorylcholine, and uracil were decreased (<0.05). The glutathione, N-acetylaspartate, phosphorylcholine and uracil in the model＋moxibustion group were significantly reversed relevant to the model group (<0.05). The inositol in gastric tissue were significantly increased (<0.05), 3-hydroxybutyric acid, serine and uridine diphosphate glucose were significantly decreased (<0.05) in the normal ＋ moxibustion group than in the normal group.
Moxibustion has a good efficacy in regulating some metabolic substances such as glutathione, N-acetylaspartic acid, choline phosphate and uracil of CAG rats, suggesting a specific relationship between the Stomach Meridian and the stomach.
观察艾灸“足三里”（ST36）对慢性萎缩性胃炎（CAG）大鼠胃组织代谢产物的影响，基于代谢组学探讨阳明经与胃的相关性。
将56只SD大鼠随机分为正常组、正常＋艾灸组、模型组和模型＋艾灸组（每组14只）。采用灌胃N-甲基-N'-硝基-N-亚硝基胍（MNNG）溶液并结合不规则饮食12周的方法建立CAG模型。正常＋艾灸组和模型＋艾灸组对“足三里”和“中脘”（CV12）进行艾灸15分钟，每日1次，共2周。通过苏木精-伊红（H.E.）染色观察胃组织的病理组织学变化。采用氢核磁共振（H NMR）波谱法检测胃组织代谢产物，并使用Chenomx NMR Suite软件对数据进行分析。
造模后，局部胃组织胃壁变薄、苍白，局部腺体减少，伴有坏死及排列紊乱，上皮细胞脱落，淋巴细胞浸润以及散在点状出血，模型＋艾灸组上述表现明显较轻。与正常组相比，模型组胃组织中乳酸、谷胱甘肽、N-乙酰天门冬氨酸、尿苷二磷酸葡萄糖和肌糖的代谢产物增加（P<0.05），亮氨酸、甜菜碱、磷酸胆碱和尿嘧啶的代谢产物减少（P<0.05）。与模型组相比，模型＋艾灸组谷胱甘肽、N-乙酰天门冬氨酸、磷酸胆碱和尿嘧啶明显逆转（P<0.05）。与正常组相比，正常＋艾灸组胃组织中肌醇明显增加（P<0.05），3-羟基丁酸、丝氨酸和尿苷二磷酸葡萄糖明显减少（P<0.05）。
艾灸对CAG大鼠谷胱甘肽、N-乙酰天门冬氨酸、磷酸胆碱和尿嘧啶等部分代谢物质具有良好的调节作用，提示胃经与胃之间存在特定关系。
