School of Food and Biological Engineering , Hefei University of Technology , Hefei 230009 , China.
School of Science , Renmin University of China , Beijing 100872 , China.
J Agric Food Chem. 2019 Apr 24;67(16):4435-4443. doi: 10.1021/acs.jafc.9b00325. Epub 2019 Apr 12.
Aspergillus niger, which is a fungal pathogen, causes rot in a variety of fruits. In this study, the cystathionine β-synthase cbsA gene was deleted by homologous recombination to study its role in sulfur metabolism and pathogenicity of A. niger. The results showed that Δ cbsA strain maintained normal mycelia growth and sporulation compared with the control strain A. niger MA 70.15, whereas the contents of cysteine and glutathione (GSH) increased significantly after cbsA deletion. However, Δ cbsA strain showed reduced endogenous HS production. Further results showed that cbsA gene deletion induced higher resistance to cadmium stress and stronger infectivity to pears. It was also found that a stronger response of reactive oxygen species (ROS) production was induced in Δ cbsA mutant-infected pear compared with the control strain. In all, the present research suggested the important role of cbsA in sulfur metabolism and pathogenicity of A. niger in pear fruit.
黑曲霉是一种真菌病原体，可导致多种水果腐烂。在这项研究中，通过同源重组删除了半胱氨酸β-合酶 cbsA 基因，以研究其在黑曲霉硫代谢和致病性中的作用。结果表明，与对照菌株黑曲霉 MA 70.15相比，ΔcbsA 菌株的菌丝生长和产孢保持正常，而 cbsA 缺失后半胱氨酸和谷胱甘肽（GSH）的含量显著增加。然而，ΔcbsA 菌株的内源性 HS 产生减少。进一步的结果表明，cbsA 基因缺失诱导了对镉胁迫的更高抗性和对梨的更强感染力。还发现，与对照菌株相比，ΔcbsA 突变体感染的梨中诱导了更强的活性氧（ROS）产生反应。总之，本研究表明 cbsA 在黑曲霉对梨果实的硫代谢和致病性中起重要作用。
