Xu Manlin, Zhang Xia, Yu Jing, Guo Zhiqing, Li Ying, Song Xinying, He Kang, Li Guowei, Chi Yucheng
Shandong Peanut Research Institute, Qingdao, China.
Institute of Crop Germplasm Resources, SAAS, Jinan, China.
Front Microbiol. 2021 Aug 18;12:719337. doi: 10.3389/fmicb.2021.719337. eCollection 2021.
is a very destructive pathogen causing severe peanut root rot, especially in the seeding stage of peanuts (), and often leading to the death of the plant. Protein lysine 2-hydroxyisobutyrylation (Khib) is a newly detected post-translational modification identified in several species. In this study, we identified 5041 Khib sites on 1,453 modified proteins in . Compared with five other species, has conserved and novel proteins. Bioinformatics analysis showed that Khib proteins are widely distributed in and are involved in many biological processes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that Khib proteins were significantly enriched in many cellular compartments and pathways, such as ribosomes and proteasome subunits. A total of 223 Khib proteins were part of the PPI network, thus, suggesting that Khib proteins are associated with a large range of protein interactions and diverse pathways in the life processes of . Several identified proteins are involved in pathogenesis regulation. Our research provides the first comprehensive report of Khib and an extensive database for potential functional studies on Khib proteins in this economically important fungus.
是一种极具破坏性的病原体，会引发严重的花生根腐病，尤其是在花生的播种阶段（），并常常导致植株死亡。蛋白质赖氨酸2-羟基异丁酰化（Khib）是在多个物种中新发现的一种翻译后修饰。在本研究中，我们在中鉴定出1453个修饰蛋白上的5041个Khib位点。与其他五个物种相比，具有保守和新颖的蛋白质。生物信息学分析表明，Khib蛋白在中广泛分布，并参与许多生物学过程。基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析显示，Khib蛋白在许多细胞区室和途径中显著富集，如核糖体和蛋白酶体亚基。共有223个Khib蛋白是蛋白质-蛋白质相互作用（PPI）网络的一部分，因此表明Khib蛋白与该重要经济真菌生命过程中的大量蛋白质相互作用和多样途径相关。一些鉴定出的蛋白质参与致病机制调控。我们的研究首次提供了关于Khib的全面报告，并为该重要经济真菌中Khib蛋白的潜在功能研究提供了一个广泛的数据库。
