Choudhary Sharda, Naika Mahantesha B N, Sharma Radheshyam, Meena R D, Singh Ravindra, Lal Gopal
ICAR-National Research Centre on Seed Spices, Tabiji, Ajmer 305 206, India.
J Genet. 2019 Mar;98.
Stem gall ( Unger), a serious disease that affects leaves, petioles, stems and fruits of coriander ( L.) causing heavy loss in yield. Genetic improvement of coriander for stem gall disease is indispensable. Coriander cultivars of stem gall resistance (ACr-1) and susceptible (CS-6) leaf samples were utilized and transcriptome sequenced using Illumina NextSeq500 platform. After trimming low-quality reads and adapter sequences, a total of 49,163,108 and 43,746,120 high-quality reads were retained and further assembly resulted validated transcripts of 59,933 and 56,861. We have predicted 52,506 and 48,858 coding sequences (CDS) ofwhich 50,506 and 46,945 were annotated using NCBInr database. Gene ontology analysis annotated 19,099 and 17,625 terms; pathway analysis obtained 24 different functional pathway categories; signal transduction, transport, catabolism, translation and carbohydrate metabolism pathways etc. were dominated. Differentially expressed genes analysis predicted 13,123 CDS commonly expressed of which 431 and 400 genes were significantly upregulated and downregulated, respectively, in which Rgenes, stress inducible transcription factors such as ERF, NAC, bZIP, MYB, DREB and WRKY and antifungal related genes were predicted. The real-time PCR analysis of HSP20 gene expression in resistance showed upregulation by 10-fold over susceptible sample and 18s used as a housekeeping gene for normalization. The present results provide an insights into various aspects underlying the development of resistance to stem gall in coriander.
茎瘿病（翁格尔）是一种严重病害，会影响芫荽（L.）的叶片、叶柄、茎和果实，导致产量大幅损失。对芫荽进行茎瘿病的遗传改良至关重要。利用了抗茎瘿病（ACr - 1）和感病（CS - 6）的芫荽品种叶片样本，并使用Illumina NextSeq500平台进行转录组测序。在去除低质量 reads 和接头序列后，分别保留了49,163,108和43,746,120条高质量 reads，进一步组装得到了59,933和56,861条经过验证的转录本。我们预测了52,506和48,858个编码序列（CDS），其中50,506和46,945个使用NCBInr数据库进行了注释。基因本体分析注释了19,099和17,625个术语；通路分析获得了24个不同的功能通路类别；信号转导、运输、分解代谢、翻译和碳水化合物代谢通路等占主导地位。差异表达基因分析预测了13,123个共同表达的CDS，其中分别有431和400个基因显著上调和下调，其中预测了R基因、应激诱导转录因子如ERF、NAC、bZIP、MYB、DREB和WRKY以及抗真菌相关基因。抗性中HSP20基因表达的实时PCR分析显示，与感病样本相比上调了10倍，18s用作管家基因进行标准化。目前的结果为芫荽抗茎瘿病发育的各个方面提供了见解。
