Lundberg Louise, Sienkiewicz Zenon, Anthony Daniel C, Broom Kerry A
Public Health England, Chilton, United Kingdom.
Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
Bioelectromagnetics. 2019 May;40(4):250-259. doi: 10.1002/bem.22188. Epub 2019 Apr 4.
Artificial light and power frequency magnetic fields are ubiquitous in the built environment. Light is a potent zeitgeber but it is unclear whether power frequency magnetic fields can influence circadian rhythm control. To study this possibility, 8-12-week-old male C57BL/6J mice were exposed for 30 min starting at zeitgeber time 14 (ZT14, 2 h into the dark period of the day) to 50 Hz magnetic fields at 580 μT using a pair of Helmholtz coils and/or a blue LED light at 700 lux or neither. Our experiments revealed an acute adrenal response to blue light, in terms of increased adrenal per1 gene expression, increased serum corticosterone levels, increased time spent sleeping, and decreased locomotor activity (in all cases, P < 0.0001) compared to an unexposed control group. There appeared to be no modulating effect of the magnetic fields on the response to light, and there was also no effect of the magnetic fields alone (in both cases, P > 0.05) except for a decrease in locomotor activity (P < 0.03). Gene expression of the cryptochromes cry1 and cry2 in the adrenals, liver, and hippocampus was also not affected by exposures (in all cases, P > 0.05). In conclusion, these results suggest that 50 Hz magnetic fields do not significantly affect the acute light response to a degree that can be detected in the adrenal response. Bioelectromagnetics. 2019;9999:XX-XX. © 2019 Bioelectromagnetics Society.
人工光和工频磁场在建筑环境中无处不在。光是一种强大的昼夜节律起搏器，但尚不清楚工频磁场是否会影响昼夜节律控制。为了研究这种可能性，将8至12周龄的雄性C57BL/6J小鼠从昼夜节律时间14（ZT14，一天黑暗期的2小时）开始，使用一对亥姆霍兹线圈暴露于580μT的50Hz磁场和/或700勒克斯的蓝色LED光下30分钟，或者不进行暴露。我们的实验显示，与未暴露的对照组相比，蓝光会引起急性肾上腺反应，表现为肾上腺per1基因表达增加、血清皮质酮水平升高、睡眠时间增加以及运动活动减少（在所有情况下，P < 0.0001）。磁场似乎对光反应没有调节作用，单独的磁场也没有影响（在两种情况下，P > 0.05），但运动活动有所减少（P < 0.03）。肾上腺、肝脏和海马体中隐花色素cry1和cry2的基因表达也不受暴露影响（在所有情况下，P > 0.05）。总之，这些结果表明，50Hz磁场不会在肾上腺反应中能检测到的程度上显著影响急性光反应。《生物电磁学》。2019；9999：XX - XX。©2019生物电磁学协会。
