Cheng Shitong, Sun Chao, Lao Wenting, Kang Hui
1 Department of Laboratory Medicine, The First Hospital of China Medical University, Shenyang, Liaoning, P.R. China.
2 Department of Clinical Laboratory Diagnostics, China Medical University, Shenyang, Liaoning, P.R. China.
Genet Test Mol Biomarkers. 2019 May;23(5):299-303. doi: 10.1089/gtmb.2018.0265. Epub 2019 Apr 4.
Autophagic eradication of pathogenic microbes, including (Mtb), is an effective host immune process that protects hosts from developing diseases associated with intracellular pathogens. This study was designed to investigate the association between the single nucleotide polymorphisms (SNPs) of the autophagy-related genes and , and the susceptibility to pulmonary tuberculosis (PTB) in a Chinese Han population. Two SNPs, rs1010 from the gene and rs15493 from the gene, were examined in 202 PTB patients and 216 healthy controls using high-resolution melt-polymerase chain reaction. The rs1010 SNP genotypes AG ( = 0.028) and GG ( = 0.016) were associated with increased susceptibility to PTB. However, the rs15493 SNP had no impact on the susceptibility to PTB ( > 0.05). Our study demonstrated that the rs1010 SNP of gene was significantly associated with the susceptibility to PTB. This result suggests that rs1010 genotyping could be used as prognostic biomarker to predict the risk of Mtb infection and/or PTB disease development after Mtb infection in the Chinese Han population.
自噬清除包括结核分枝杆菌（Mtb）在内的致病微生物是一种有效的宿主免疫过程，可保护宿主免受与细胞内病原体相关疾病的侵害。本研究旨在探讨自噬相关基因和的单核苷酸多态性（SNP）与中国汉族人群肺结核（PTB）易感性之间的关联。使用高分辨率熔解-聚合酶链反应对202例PTB患者和216例健康对照进行了基因的rs1010和基因的rs15493这两个SNP的检测。rs1010 SNP的AG（=0.028）和GG（=0.016）基因型与PTB易感性增加相关。然而，rs15493 SNP对PTB易感性没有影响（>0.05）。我们的研究表明，基因的rs1010 SNP与PTB易感性显著相关。这一结果表明，rs1010基因分型可作为预测中国汉族人群Mtb感染风险和/或Mtb感染后PTB疾病发生风险的预后生物标志物。
