Duan Cheng-cheng, Xu Li-xin, Chen Qian
Department of Cardiovascular Medicine, Shenzhen Shajing Affiliated Hospital, Guangzhou Medical University, Shenzhen, Guangdong, 518104 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Jun;27(3):329-32. doi: 10.3760/cma.j.issn.1003-9406.2010.0.020.
To investigate the association of synaptobrevins/vesicle-associated membrane proteins 8 (VAMP8) gene rs1010 polymorphism with coronary heart disease (CHD) in Chinese Han population.
The allele and genotype frequencies of the VAMP8 gene rs1010 locus in 185 CHD patients and 149 controls were analyzed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing.
There was polymorphism of the VAMP8 gene rs1010 locus in the studied population. The distribution of VAMP8 genotypes was in Hardy-Weinberg equilibrium. The frequency of the A allele in the CHD group was significantly higher than that in control (67.3% vs 53.0%, P< 0.05). Multiple logistic regression analysis showed that genotypes AA and AG were independent risk factors of coronary heart disease. The odds ratio (OR) of (AA+AG) genotype versus GG genotype was 1.969,95% CI: 1.032-3.755.
The VAMP8 rs1010 polymorphism was associated with CHD risk in Chinese Han population, the A allele might serve as a genetic risk factor of coronary heart disease.
探讨突触小泡蛋白/小泡相关膜蛋白8（VAMP8）基因rs1010多态性与中国汉族人群冠心病（CHD）的相关性。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和DNA测序技术，分析185例冠心病患者和149例对照者VAMP8基因rs1010位点的等位基因和基因型频率。
研究人群中VAMP8基因rs1010位点存在多态性。VAMP8基因型分布符合Hardy-Weinberg平衡。冠心病组A等位基因频率显著高于对照组（67.3%比53.0%，P<0.05）。多因素logistic回归分析显示，AA和AG基因型是冠心病的独立危险因素。（AA+AG）基因型与GG基因型的比值比（OR）为1.969，95%可信区间：1.032-3.755。
VAMP8 rs1010多态性与中国汉族人群冠心病风险相关，A等位基因可能是冠心病的遗传危险因素。
