Gray D W, Warnock G L, Sutton R, Peters M, McShane P, Morris P J
Br J Surg. 1986 Oct;73(10):850-3. doi: 10.1002/bjs.1800731029.
A recently described method for isolation of islets of Langerhans from the human pancreas has been adapted to the cynomolgus monkey pancreas. The mean yield of islets obtained from 13 monkey pancreata was 1319 islets per gram of pancreatic tissue (range 533-1800) and the usual purity of the preparation varied from 5 to 15 per cent islet tissue. Twelve cynomolgus monkeys underwent total pancreatectomy, preparation of islets from the excised pancreas and autotransplantation to either the spleen or the liver. Three animals received no transplant and became immediately diabetic, surviving 4-8 days. Four animals became normoglycaemic after intrasplenic islet transplantation, and survived 6 weeks, at which time splenectomy was performed with immediate onset of diabetes. Splenic vein insulin sampling confirmed the spleen as the source of insulin, and histological examination showed implanted islet tissue in all cases. Five animals became normoglycaemic after intrahepatic islet implantation. Three animals subsequently became diabetic at 4 and 5 months and two animals still have functioning grafts, the longest function being 9 months. These results suggest that in the cynomolgus monkey sufficient islets can be extracted from a single donor pancreas to reverse diabetes.
一种最近描述的从人胰腺中分离朗格汉斯岛的方法已被应用于食蟹猴胰腺。从13个猴胰腺中获得的胰岛平均产量为每克胰腺组织1319个胰岛（范围为533 - 1800个），且制备物的通常纯度为胰岛组织的5%至15%。12只食蟹猴接受了全胰切除术，从切除的胰腺中制备胰岛并自体移植到脾脏或肝脏。3只动物未接受移植，立即出现糖尿病，存活4 - 8天。4只动物在脾内胰岛移植后血糖恢复正常，存活6周，此时进行脾切除术，随后立即出现糖尿病。脾静脉胰岛素采样证实脾脏是胰岛素的来源，组织学检查显示所有病例均有植入的胰岛组织。5只动物在肝内胰岛植入后血糖恢复正常。3只动物随后在4个月和5个月时出现糖尿病，2只动物的移植物仍有功能，最长功能持续9个月。这些结果表明，在食蟹猴中，从单个供体胰腺中可以提取足够的胰岛来逆转糖尿病。
