Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia; Department of Sciences, College of Science & Technology, Waziri Umaru Federal Polytechnic, Birnin Kebbi, Nigeria.
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia.
Genomics. 2020 Jan;112(1):484-493. doi: 10.1016/j.ygeno.2019.03.011. Epub 2019 Apr 1.
Exposing the skin to solar UV radiation induces cascades of signaling pathways and biological alterations such as redox imbalance, suppression of antioxidant genes and programmed cell death. Therefore, the aim of this study was to use RNA-Seq to unravel the effects of UV radiation on Normal Human Adult Fibroblast cells (NHDF). Cells were exposed to UV (20 mJ/cm2 for 3 mins) and incubated for 24 h. Total mRNA from the cells generated libraries of 72,080,648 and 40,750,939 raw reads from UV-treated and control cells respectively. Of the differentially expressed genes (DEGs) produced 2,007 were up-regulated and 2,791 were down-regulated (fold change ≥2, p < 0.05). The expression of 4 genes was validated with RT-qPCR. Chemokine signaling pathways in cancer were significantly activated and antioxidant genes were down-regulated. This study applied Next Generation Sequencing technology to reveal the genes and pathways involved in UV-induced human dermal fibroblast cells necrosis.
暴露于太阳紫外线辐射会引发信号通路级联反应和生物学改变，如氧化还原失衡、抗氧化基因抑制和程序性细胞死亡。因此，本研究旨在使用 RNA-Seq 揭示紫外线对正常人类成纤维细胞（NHDF）的影响。将细胞暴露于紫外线（20mJ/cm2，3 分钟）并孵育 24 小时。来自紫外线处理和对照细胞的总 mRNA 分别生成了 72080648 和 40750939 个原始读数的文库。产生的差异表达基因（DEGs）中，有 2007 个上调，2791 个下调（倍数变化≥2，p<0.05）。用 RT-qPCR 验证了 4 个基因的表达。癌症中的趋化因子信号通路明显激活，抗氧化基因下调。本研究应用下一代测序技术揭示了紫外线诱导人真皮成纤维细胞坏死涉及的基因和通路。
