Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
Bull Exp Biol Med. 2023 Apr;174(6):707-710. doi: 10.1007/s10517-023-05775-5. Epub 2023 May 8.
We studied the effects of age and suntan on the expression of necroptosis signaling molecules (RIPK1, RIPK3, and MLKL kinases) and first TNF receptor (TNFR1) in isolated skin cells from women undergoing facelift surgery. In women above 50 years, the expression of the TNFR1, kinases RIPK1, RIPK3, and MLKL, the phosphorylated forms of these kinases was significantly (p<0.05) increased in comparison with the corresponding parameters in women under 30 years. The expression of all necroptosis proteins and TNFR1 in women with suntan was significantly (p<0.05) higher than in those without tan. Cells from the surgical material were incubated with TNFα to determine the level of induced necroptosis. In women aged >50 years and women with suntan, the expression of phosphorylated forms of kinases was significantly increased, which attested to necroptosis activation. This study allowed identifying the targets on skin cells for prevention of necrosis and inflammation after facelift surgery.
我们研究了年龄和晒黑对接受面部拉皮手术的女性分离皮肤细胞中坏死信号分子（RIPK1、RIPK3 和 MLKL 激酶）和第一 TNF 受体（TNFR1）表达的影响。在 50 岁以上的女性中，与 30 岁以下女性相比，TNFR1、激酶 RIPK1、RIPK3 和 MLKL 及其磷酸化形式的表达显著增加（p<0.05）。所有坏死蛋白和 TNFR1 在有晒黑的女性中的表达显著高于没有晒黑的女性（p<0.05）。用 TNFα 孵育手术材料中的细胞以确定诱导的坏死程度。在年龄>50 岁的女性和有晒黑的女性中，激酶的磷酸化形式的表达显著增加，这证明了坏死的激活。这项研究确定了皮肤细胞的靶点，以预防面部拉皮手术后的坏死和炎症。
