Department of General and Environmental Microbiology, Poznań University of Life Sciences, Szydłowska 50, 60-656 Poznań, Poland.
Institute of Biosystems Engineering, Poznań University of Life Sciences, Wojska Polskiego 50, 60-656 Poznań, Poland.
Sci Total Environ. 2019 Jun 25;671:795-804. doi: 10.1016/j.scitotenv.2019.03.120. Epub 2019 Mar 9.
It has been assumed that compost from savoy cabbage and rapeseed straw is a good substrate for discrimination of the reproduction potential of Trichoderma strains. This hypothesis was verified based on a two-stage incubation experiment. The prepared mixture was fermented in a bio-reactor for 11 weeks. In the second experiment, the mature compost was inoculated with four strains of Trichoderma and a spore concentration of 10 and 10, and then incubated for four weeks. The biomass of autogenic fungi reached a maximum of 12.5 mg∙g DM in the cooling phase. The variability in temperature during composting significantly affected NH emission. The pH of mature compost from cabbage wastes, as a result of the elevated NH emission reached the alkaline range. The survival of the Trichoderma fungi introduced into the alkali substrate was a result of strain sensitivity to the high pH of the compost and to the initial inoculum density. The adaptation potential of Trichoderma harzianum to the alkali milieu depended on the pH stabilization of the substrate by this fungi, provided the spore inoculum density was 10. The strains of Trichoderma atroviride responded negatively, regardless of the inoculum density, to the alkaline pH of the substrate and to self-induced changes in the compost pH.
人们一直认为,来自皱叶甘蓝和油菜秸秆的堆肥是区分木霉菌株繁殖潜力的良好基质。这一假设是基于两阶段的孵化实验来验证的。准备好的混合物在生物反应器中发酵 11 周。在第二个实验中,成熟的堆肥接种了四种木霉菌株,孢子浓度为 10 和 10,然后培养四周。在冷却阶段,自生真菌的生物量达到最大值 12.5mg·g DM。堆肥过程中温度的变化显著影响了 NH 的排放。由于 NH 排放的增加,来自甘蓝废弃物的成熟堆肥的 pH 值达到了碱性范围。在碱性基质中引入的木霉真菌的存活是由于菌株对堆肥高 pH 值和初始接种密度的敏感性所致。木霉 harzianum 对碱性环境的适应能力取决于该真菌对基质 pH 的稳定作用,前提是孢子接种密度为 10。无论接种密度如何,深绿木霉对基质的碱性 pH 和堆肥自身 pH 的变化都有负面反应。