Ros Margarita, Raut Iulia, Santisima-Trinidad Ana Belén, Pascual Jose Antonio
Department of Soil and Water Conservation and Organic Waste Management, Centro de Edafología y Biología Aplicada del Segura, CSIC, Murcia, Spain.
National institute for Research & Development in Chemistry & Petrochemistry - ICECHIM, Biotechnology & Bioanalysis group, Bucharest, Romania.
PLoS One. 2017 Mar 27;12(3):e0174069. doi: 10.1371/journal.pone.0174069. eCollection 2017.
The understanding of the dynamic of soil-borne diseases is related to the microbial composition of the rhizosphere which is the key to progress in the field of biological control. Trichoderma spp. is commonly used as a biological control agent. The use of next generation sequencing approaches and quantitative PCR are two successful approaches to assess the effect of using compost as substrate fortified with two Trichoderma strains (Trichoderma harzianum or Trichoderma asperellum) on bacterial and fungal communities in pepper rhizosphere infected with Phytophthora nicotianae. The results showed changes in the bacterial rhizosphere community not attributed to the Trichoderma strain, but to the pathogen infection, while, fungi were not affected by pathogen infection and depended on the type of substrate. The Trichoderma asperellum fortified compost was the most effective combination against the pathogen. This could indicate that the effect of fortified composts is greater than compost itself and the biocontrol effect should be attributed to the Trichoderma strains rather than the compost microbiota, although some microorganisms could help with the biocontrol effect.
对土传病害动态的理解与根际微生物组成有关，而根际微生物组成是生物防治领域取得进展的关键。木霉菌属通常用作生物防治剂。使用新一代测序方法和定量PCR是两种成功的方法，用于评估以两种木霉菌株（哈茨木霉或棘孢木霉）强化的堆肥作为基质，对感染烟草疫霉的辣椒根际细菌和真菌群落的影响。结果表明，细菌根际群落的变化并非归因于木霉菌株，而是归因于病原体感染，而真菌不受病原体感染的影响，且取决于基质类型。用棘孢木霉强化的堆肥是对抗病原体最有效的组合。这可能表明，强化堆肥的效果大于堆肥本身，生物防治效果应归因于木霉菌株而非堆肥微生物群，尽管一些微生物可能有助于生物防治效果。
