Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290, Châtenay-Malabry, France.
University of Basel, Department of Chemistry, Spitalstrasse 51, 4056, Basel, Switzerland.
Anal Chim Acta. 2019 Jul 25;1062:156-164. doi: 10.1016/j.aca.2019.02.006. Epub 2019 Feb 21.
This study reports on the conception of magneto-Capillary Electrophoresis (magneto-CE), an approach integrating immuno-capture on circulating bio-functionalized magnetic beads into a unique capillary for preconcentration and electrokinetic separation. This hybrid mode is an evolution of in-capillary magnetic bead-based operation from static cluster format to dynamic configuration where beads are allowed to controllably circulate inside a CE capillary for interaction improvement. To implement the magneto-CE operation, a purpose-made instrument was constructed, allowing visual observation of the movement of the magnetic beads. We applied a new methodological strategy for determination of the amyloid β peptide (Aβ 1-42), which is as an established biomarker for molecular diagnosis of Alzheimer's disease (AD). The methodology is based on magneto-immuno-capture of fluorescently labeled Aβ 1-42 followed by a chemical elution with a basic solution prior to CE separation with laser induced fluorescent (LIF) detection. The superiority of this dynamic configuration of magneto-CE was demonstrated for this target analyte, with sample pretreatment and separation being performed in-capillary without any delay in between and without any waste of pretreated sample, which otherwise would not be the case with offline/batch-wise operation.
本研究报告了磁控毛细管电泳（magneto-CE）的构想，该方法将循环生物功能化磁性珠上的免疫捕获整合到独特的毛细管中，用于预浓缩和电动分离。这种混合模式是在毛细管内基于磁性珠操作的演变，从静态簇格式到动态配置，允许珠子在 CE 毛细管内可控地循环以改善相互作用。为了实施磁控 CE 操作，构建了一种专用仪器，可以观察到磁性珠的运动。我们应用了一种新的方法策略来测定淀粉样β肽（Aβ 1-42），这是阿尔茨海默病（AD）分子诊断的一种既定生物标志物。该方法基于荧光标记的 Aβ 1-42 的磁免疫捕获，然后用碱性溶液进行化学洗脱，再进行 CE 分离，用激光诱导荧光（LIF）检测。对于该目标分析物，这种动态磁控 CE 配置的优越性得到了证明，样品预处理和分离在毛细管内进行，无需任何延迟，也不会浪费预处理的样品，而离线/分批操作则不然。
