采用毛细管瞬变等速电泳技术对不同纵横比和尺寸的金纳米材料进行高分离效率的分离。
High separation efficiency of gold nanomaterials of different aspect ratio and size using capillary transient isotachophoresis.
机构信息
National Institute of Standards and Technology, Material Measurement Laboratory - Chemical Sciences Division, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
National Institute of Standards and Technology, Material Measurement Laboratory - Materials Measurement Science Division, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
出版信息
J Chromatogr A. 2019 Aug 2;1598:216-222. doi: 10.1016/j.chroma.2019.03.054. Epub 2019 Mar 25.
Two modes of capillary electrophoresis (CE), capillary zone electrophoresis (CZE) and capillary transient isotachophoresis (ctITP), were compared for the detection and separation of spherical gold nanoparticles (AuNPs) and gold nanorods (AuNRs). The development of ctITP using two different leading ions is described. Overall, when compared to traditional capillary zone electrophoresis (CZE), ctITP resulted in improved peak shape and peak efficiency. Specifically, the number of theoretical plates for AuNR samples increased by a factor of 2-2.5 depending on the choice of leading ion. Further, using ctITP two AuNRs differing by aspect ratio were baseline resolved, whereas the same AuNRs could not be separated using CZE or other techniques like single particle inductively coupled plasma mass spectrometry (spICP-MS) and asymmetric flow field-flow fractionation (AF4). The results of this study demonstrate that ctITP is an efficient on-line technique for the improved detection and separation of gold nanomaterials in CE.
两种毛细管电泳模式（CE），即毛细管区带电泳（CZE）和毛细管瞬态等速电泳（ctITP），被用于检测和分离球形金纳米粒子（AuNPs）和金纳米棒（AuNRs）。描述了使用两种不同先导离子的 ctITP 的发展。总的来说，与传统的毛细管区带电泳（CZE）相比，ctITP 导致了更好的峰形和更高的峰效率。具体而言，根据先导离子的选择，AuNR 样品的理论塔板数增加了 2-2.5 倍。此外，使用 ctITP 可以基线分离两种不同纵横比的 AuNRs，而使用 CZE 或其他技术，如单颗粒电感耦合等离子体质谱（spICP-MS）和不对称流场流分离（AF4），则无法分离相同的 AuNRs。本研究的结果表明，ctITP 是一种有效的在线技术，可用于提高 CE 中金纳米材料的检测和分离效率。
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