Riley Kathryn R, Sims Christopher M, Wood Imani T, Vanderah David J, Walker Marlon L
Material Measurement Laboratory, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA.
Department of Chemistry and Biochemistry, Swarthmore College, Swarthmore, PA, 19081, USA.
Anal Bioanal Chem. 2018 Jan;410(1):145-154. doi: 10.1007/s00216-017-0704-0. Epub 2017 Oct 30.
Protein corona formed on nanomaterial surfaces play an important role in the bioavailability and cellular uptake of nanomaterials. Modification of surfaces with oligoethylene glycols (OEG) are a common way to improve the resistivity of nanomaterials to protein adsorption. Short-chain ethylene oxide (EO) oligomers have been shown to improve the protein resistance of planar Au surfaces. We describe the application of these EO oligomers for improved protein resistance of 30 nm spherical gold nanoparticles (AuNPs). Functionalized AuNPs were characterized using UV-Vis spectroscopy, dynamic light scattering (DLS), and zeta potential measurements. Capillary electrophoresis (CE) was used for separation and quantitation of AuNPs and AuNP-protein mixtures. Specifically, nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) was employed for the determination of equilibrium and rate constants for binding between citrate-stabilized AuNPs and two model proteins, lysozyme and fibrinogen. Semi-quantitative CE analysis was carried out for mixtures of EO-functionalized AuNPs and proteins, and results demonstrated a 2.5-fold to 10-fold increase in protein binding resistance to lysozyme depending on the AuNP surface functionalization and a 15-fold increase in protein binding resistance to fibrinogen for both EO oligomers examined in this study. Graphical abstract Using capillary electrophoresis, the addition of short-chained oligo(ethylene oxide) ligands to gold nanoparticles was shown to improve protein binding resistance up to 15-fold.
在纳米材料表面形成的蛋白质冠层在纳米材料的生物利用度和细胞摄取中起着重要作用。用低聚乙二醇（OEG）修饰表面是提高纳米材料对蛋白质吸附抗性的常用方法。短链环氧乙烷（EO）低聚物已被证明可提高平面金表面的抗蛋白质能力。我们描述了这些EO低聚物在提高30nm球形金纳米颗粒（AuNP）抗蛋白质能力方面的应用。使用紫外可见光谱、动态光散射（DLS）和zeta电位测量对功能化的AuNP进行了表征。毛细管电泳（CE）用于AuNP和AuNP-蛋白质混合物的分离和定量。具体而言，平衡混合物的非平衡毛细管电泳（NECEEM）用于测定柠檬酸盐稳定的AuNP与两种模型蛋白质（溶菌酶和纤维蛋白原）之间结合的平衡常数和速率常数。对EO功能化的AuNP与蛋白质的混合物进行了半定量CE分析，结果表明，根据AuNP表面功能化情况，对溶菌酶的蛋白质结合抗性提高了2.5倍至10倍，本研究中检测的两种EO低聚物对纤维蛋白原的蛋白质结合抗性提高了15倍。图形摘要 使用毛细管电泳，向金纳米颗粒中添加短链聚环氧乙烷配体可将蛋白质结合抗性提高多达15倍。
