İzmir Institute of Technology, Graduate Program of Biotechnology and Bioengineering, Gülbahçe Campus, Urla 35430, İzmir, Turkey; İzmir Institute of Technology, Department of Chemical Engineering, Gülbahçe Campus, Urla 35430, İzmir, Turkey.
İzmir Institute of Technology, Department of Chemical Engineering, Gülbahçe Campus, Urla 35430, İzmir, Turkey.
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:196-208. doi: 10.1016/j.msec.2019.02.104. Epub 2019 Mar 1.
Recently, natural polymers are reinforced with silica particles for hard tissue engineering applications to induce bone regeneration. In this study, as two novel bioactive agents, effects of diatomite and polyhedral oligomeric silsesquioxanes (POSS) on chitosan (CS)/Na-carboxymethylcellulose (Na-CMC) polymer blend scaffolds are examined. In addition, the effect of silica reinforcements was compared with Si-substituted nano-hydroxyapatite (Si-Hap) particles. The morphology, physical and chemical structures of the scaffolds were characterized with SEM, liquid displacement, FT-IR, mechanical analysis, swelling and degradation studies. The particle size and the crystal structure of diatomite, POSS and Si-Hap particles were determined with DLS and XRD analyses. In vitro studies were performed to figure out the cytotoxicity, proliferation, ALP activity, osteocalcin production and biomineralization to demonstrate the promising use of natural silica particles in bone regeneration. Freeze-dried scaffolds showed 190-307 μm pore size range and 61-70% porosity. Both inorganic reinforcements increased the mechanical strength, enhanced the water uptake capacity and fastened the degradation rate. The nanocomposite scaffolds did not show any cytotoxic effect and enhanced the surface mineralization in osteogenic medium. Thus, diatomite and POSS cage structures can be potential reinforcements for nanocomposite design in hard tissue engineering applications.
最近，天然聚合物与硅颗粒结合用于硬组织工程应用，以诱导骨再生。在这项研究中，作为两种新型生物活性物质，探讨了硅藻土和多面体低聚倍半硅氧烷（POSS）对壳聚糖（CS）/羧甲基纤维素钠（Na-CMC）聚合物共混支架的影响。此外，还比较了硅增强材料与硅取代纳米羟基磷灰石（Si-Hap）颗粒的效果。采用 SEM、液体置换、FT-IR、力学分析、溶胀和降解研究对支架的形态、物理和化学结构进行了表征。采用 DLS 和 XRD 分析确定了硅藻土、POSS 和 Si-Hap 颗粒的粒径和晶体结构。进行了体外研究以确定细胞毒性、增殖、碱性磷酸酶（ALP）活性、骨钙素产生和生物矿化作用，以证明天然硅颗粒在骨再生中的应用前景。冷冻干燥支架的孔径范围为 190-307μm，孔隙率为 61-70%。两种无机增强材料均提高了机械强度，增强了吸水性，并加快了降解速度。纳米复合材料支架没有显示出任何细胞毒性作用，并在成骨培养基中增强了表面矿化作用。因此，硅藻土和 POSS 笼状结构可以成为硬组织工程应用中纳米复合材料设计的潜在增强材料。
