State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
State Key Laboratory of Biotherapy and Laboratory, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, China.
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:505-513. doi: 10.1016/j.msec.2019.03.007. Epub 2019 Mar 3.
Biomaterials based on the modulation of macrophages have gained increased attention recently. Macrophages are generally divided into the pro-inflammatory M1 and pro-regenerative M2 phenotypes. Macrophages play a pivotal role in bone regeneration by regulating osteoblast differentiation and secreting pro-regenerative factors. In the present study, epigallocatechin-3-gallate (EGCG)-modified collagen membranes downregulated the expression of inflammatory factors and promoted the recruitment of M2 macrophages, as evidenced by the expression of M2 macrophage markers (CD163 and CD206). It is further demonstrated that the recruitment of M2 macrophages may be involved with CC chemokine receptor type 2 (CCR2) signaling, with a significant downregulation of CD206 following CCR2 knockout. These results suggested that EGCG-modified collagen membranes may modulate the recruitment of macrophages and can be applied to guided bone regeneration and guided tissue regeneration.
基于巨噬细胞调控的生物材料最近受到了越来越多的关注。巨噬细胞通常分为促炎的 M1 表型和促修复的 M2 表型。巨噬细胞通过调节成骨细胞分化和分泌促修复因子，在骨再生中发挥关键作用。在本研究中，表没食子儿茶素没食子酸酯（EGCG）修饰的胶原膜下调了炎症因子的表达，并促进了 M2 巨噬细胞的募集，这表现在 M2 巨噬细胞标志物（CD163 和 CD206）的表达上。进一步的研究表明，M2 巨噬细胞的募集可能与 C 型趋化因子受体 2（CCR2）信号有关，CCR2 敲除后 CD206 显著下调。这些结果表明，EGCG 修饰的胶原膜可能调节巨噬细胞的募集，可应用于引导性骨再生和引导组织再生。
