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一种独特的ATP酶，ArtR（PA4595），抑制Ⅲ型分泌系统。

A Unique ATPase, ArtR (PA4595), Represses the Type III Secretion System in .

作者信息

Kong Weina, Dong Mengmeng, Yan Rong, Liang Qingqing, Zhang Huiqun, Luo Wei, Zhang Yani, Liang Haihua, Duan Kangmin

机构信息

Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an, China.

Department of Oral Biology and Department of Medical Microbiology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.

出版信息

Front Microbiol. 2019 Mar 21;10:560. doi: 10.3389/fmicb.2019.00560. eCollection 2019.

DOI:10.3389/fmicb.2019.00560
PMID:30949153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6437102/
Abstract

is an important human pathogen which uses the type III secretion system (T3SS) as a primary virulence factor to establish infections in humans. The results presented in this report revealed that the ATP-binding protein PA4595 (named ArtR, a Regulator that is an ATP-activated Repressor of T3SS) represses T3SS expression in . The expression of T3SS genes, including , , , and -, increased significantly when was knockout. The effect of ArtR on ExsA is at the transcriptional level, not at the translational level. The regulatory role and cytoplasm localization of ArtR suggest it belongs to the REG sub-family of ATP-binding cassette (ABC) family. Purified GST-tagged ArtR showed ATPase activity . The conserved aspartate residues in the dual Walker B motifs prove to be essential for the regulatory function of ArtR. The regulation of T3SS by ArtR is unique, which does not involve the known GacS/A-RsmY/Z-RsmA-ExsA pathway or Vfr. This is the first REG subfamily of ATP-binding cassette that is reported to regulate T3SS genes in bacteria. The results specify a novel player in the regulatory networks of T3SS in .

摘要

是一种重要的人类病原体，它利用III型分泌系统（T3SS）作为主要毒力因子在人类中建立感染。本报告中的结果表明，ATP结合蛋白PA4595（命名为ArtR，一种作为T3SS的ATP激活阻遏物的调节因子）在中抑制T3SS表达。当被敲除时，包括、、和 - 在内的T3SS基因的表达显著增加。ArtR对ExsA的作用是在转录水平，而不是在翻译水平。ArtR的调节作用和细胞质定位表明它属于ATP结合盒（ABC）家族的REG亚家族。纯化的GST标签ArtR显示出ATP酶活性。双Walker B基序中的保守天冬氨酸残基被证明对ArtR的调节功能至关重要。ArtR对T3SS的调节是独特的，它不涉及已知的GacS/A - RsmY/Z - RsmA - ExsA途径或Vfr。这是第一个被报道在细菌中调节T3SS基因的ATP结合盒REG亚家族。这些结果明确了中T3SS调节网络中的一个新成员。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/16fd58173257/fmicb-10-00560-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/02b3e41e9e2f/fmicb-10-00560-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/17219b200da4/fmicb-10-00560-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/941564601ca4/fmicb-10-00560-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/3736aac130f0/fmicb-10-00560-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/bc3b784b9ad0/fmicb-10-00560-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/45d405629314/fmicb-10-00560-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/16fd58173257/fmicb-10-00560-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/02b3e41e9e2f/fmicb-10-00560-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/17219b200da4/fmicb-10-00560-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/941564601ca4/fmicb-10-00560-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/3736aac130f0/fmicb-10-00560-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/bc3b784b9ad0/fmicb-10-00560-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/45d405629314/fmicb-10-00560-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e44/6437102/16fd58173257/fmicb-10-00560-g007.jpg

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