Yamaguchi H
Cell Calcium. 1986 Aug;7(4):203-19. doi: 10.1016/0143-4160(86)90001-1.
Novel, double-barrelled Ca2+-selective microelectrodes with tip diameters of approximately 0.1 micron were constructed by using Simon's neutral Ca2+ ligand (ETH 1001). Concentric micropipettes were utilized for the first time for Ca2+-selective microelectrodes in which the Ca2+ ligand was incorporated into a protruding inner pipette, surrounded by an outer reference electrode. In addition, they were made from high resistance aluminosilicate glass tubing (Corning Code 1724). These Ca2+-selective electrodes had linear responses from pCa 3 to pCa 7 in the presence of constant [K+]. They provided on-line observation of changes in intracellular [Ca2+] and in the resting membrane potential in single smooth muscle cells isolated from toad stomach. The mean concentration of intracellular Ca2+ in resting cells was 163.6 +/- 20 nM (+/- SEM, n = 16). Doubling the intracellular Ca2+ level by exposure of cells to elevated [K+] was sufficient to cause shortening.
使用西蒙中性钙离子配体（ETH 1001）构建了新型双管钙离子选择性微电极，其尖端直径约为0.1微米。同心微吸管首次用于钙离子选择性微电极，其中钙离子配体被掺入突出的内部微吸管中，周围是外部参比电极。此外，它们由高电阻铝硅酸盐玻璃管（康宁代码1724）制成。在[K+]恒定的情况下，这些钙离子选择性电极在pCa 3至pCa 7范围内具有线性响应。它们提供了对从蟾蜍胃分离的单个平滑肌细胞内[Ca2+]变化和静息膜电位的在线观察。静息细胞内钙离子的平均浓度为163.6 +/- 20 nM（+/- SEM，n = 16）。通过将细胞暴露于升高的[K+]使细胞内钙离子水平加倍足以导致细胞缩短。
