Ammann D
Cell Calcium. 1985 Apr;6(1-2):39-55. doi: 10.1016/0143-4160(85)90033-8.
Ca2+-selective microelectrodes based on the synthetic neutral carrier ETH 1001 can be used for quantitative intracellular measurements of resting Ca2+-activities and of slowly changing Ca2+-levels (response time in the order of seconds). Microelectrodes with tip diameters greater than 0.3 micron show selectivities that yield a detection limit between 10(-8) and 10(-7) M Ca2+ in an intracellular background. The Ca2+-activity is obtained together with electrical parameters of the cell (e.g. cell membrane potential and membrane resistance or conductivity). Simultaneous monitoring of other ion-activities is accessible (double- or multi-barrelled microelectrodes). The Ca2+-determination is extremely local, i.e. it probably does not indicate an averaged cytosolic activity in every situation (e.g. localized transients).
基于合成中性载体ETH 1001的钙离子选择性微电极可用于对静息钙活性和缓慢变化的钙水平进行细胞内定量测量（响应时间以秒计）。尖端直径大于0.3微米的微电极在细胞内背景下的选择性可产生10^(-8)至10^(-7) M钙离子的检测限。钙离子活性与细胞的电学参数（如细胞膜电位、膜电阻或电导率）同时获得。还可以同时监测其他离子活性（双管或多管微电极）。钙离子测定具有极高的局部性，即它可能并非在每种情况下都能指示平均的胞质活性（例如局部瞬变）。
