Orlowski Dariusz, Glud Andreas N, Palomero-Gallagher Nicola, Sørensen Jens Christian H, Bjarkam Carsten R
Center for Experimental Neuroscience (Cense), Institute of Clinical Medicine - The Department of Neurosurgery, Aarhus University, Aarhus Universitetshospital, Palle Juul-Jensens Boulevard 165, Indgang J, Plan 1, J118-125, DK-8200 Aarhus N, Denmark.
Institute of Neuroscience and Medicine (INM-1), Research Centre Jülich, 52425 Jülich, Germany.
Heliyon. 2019 Mar 19;5(3):e01363. doi: 10.1016/j.heliyon.2019.e01363. eCollection 2019 Mar.
The cytoarchitecture of the Göttingen minipig telencephalon has recently been elucidated in the published article (Bjarkam et al., 2017). The aim of the current paper is to describe how such data can be presented in an online histological atlas of the Gottingen minipig brain and how this atlas was constructed.
Two sets of histological sections were used. One set was photographed in high resolution and labelled, the other set in low resolution (resized first set) was used for reference on the computer screen. The two sets of microphotographs enable, using the freely available JQuery Image Zoom Plugin, the subsequent construction of a simple HTML-based atlas web page with a "virtual microscope like" style, which allowed magnifying of the base image (low-resolution image) up to the maximum resolution of the high-resolution image. In addition, we describe how the established histological atlas can be accompanied by a set of similar T1-weighted MRI pictures.
Histological and MRI pictures are presented in atlas form on www.cense.dk/minipig_atlas/index.html. The described pipeline represent a cheap and freely available way to present histological images, in online virtual microscopic atlas form, and may thus be of general interest to anyone who would like to present histological data accordingly.
哥廷根小型猪端脑的细胞结构最近已在已发表的文章中得到阐明（Bjarkam等人，2017年）。本文的目的是描述如何在哥廷根小型猪脑的在线组织学图谱中呈现此类数据以及该图谱是如何构建的。
使用了两组组织学切片。一组以高分辨率拍摄并标记，另一组低分辨率（对第一组进行尺寸调整）用于在计算机屏幕上作为参考。这两组显微照片借助免费的JQuery图像缩放插件，随后构建了一个具有“虚拟显微镜样”风格的基于HTML的简单图谱网页，该网页允许将基础图像（低分辨率图像）放大至高分辨率图像的最大分辨率。此外，我们描述了如何为已建立的组织学图谱配上一组类似的T1加权MRI图片。
组织学和MRI图片以图谱形式呈现于www.cense.dk/minipig_atlas/index.html。所描述的流程代表了一种以在线虚拟显微镜图谱形式呈现组织学图像的廉价且免费的方式，因此可能会受到任何希望以相应方式呈现组织学数据的人的普遍关注。
