Robinson Kenneth N, Steven Rory T, Race Alan M, Bunch Josephine
National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Teddington, UK.
Advanced Materials and Healthcare Technologies Division (AMHT), University of Nottingham, Nottingham, UK.
J Am Soc Mass Spectrom. 2019 Jul;30(7):1284-1293. doi: 10.1007/s13361-019-02193-8. Epub 2019 Apr 4.
Ultraviolet matrix-assisted laser desorption/ionization mass spectrometry imaging (UV-MALDI MSI) is a widely used technique for imaging molecular distributions within biological systems. While much work exists concerning desorption in UV-MALDI MS, the effects of commonly varied parameters for imaging applications (repetition rate, use of continuous raster mode and raster speed), which determine spatial resolution and limits of detection for the technique, remain largely unknown. We use multiple surface characterization modalities to obtain quantitative measurements of material desorption and analyte ion yield in thin film model systems of two matrix compounds, arising from different UV-MALDI MSI sampling conditions. Observed changes in resulting ablation feature point to matrix-dependent spatial resolution and laser-induced matrix modification effects. Analyte ion yields of 10 to 10 are observed. Complex changes in ion yield, between spot and raster sampling and arising from varied laser repetition rate and raster speed, are observed. Graphical Abstract.
紫外基质辅助激光解吸/电离质谱成像（UV-MALDI MSI）是一种广泛应用于生物系统内分子分布成像的技术。虽然关于UV-MALDI MS中的解吸已有大量研究，但对于成像应用中常用的变化参数（重复频率、连续光栅模式的使用和光栅速度）的影响，该技术的空间分辨率和检测限在很大程度上仍不清楚。我们使用多种表面表征方法，在两种基质化合物的薄膜模型系统中，通过不同的UV-MALDI MSI采样条件，获得材料解吸和分析物离子产率的定量测量结果。观察到的烧蚀特征变化表明存在基质依赖性空间分辨率和激光诱导的基质改性效应。观察到分析物离子产率在10至10之间。在点采样和光栅采样之间以及由于不同的激光重复频率和光栅速度而产生的离子产率的复杂变化也被观察到。图形摘要。
