Departement für Chemie und Biochemie, Universität Bern, CH-3012 Bern, Switzerland; email:
Annu Rev Anal Chem (Palo Alto Calif). 2016 Jun 12;9(1):365-85. doi: 10.1146/annurev-anchem-071015-041750. Epub 2016 Apr 6.
The coupled physical and chemical dynamics model of ultraviolet matrix-assisted laser desorption/ionization (MALDI) has reproduced and explained a wide variety of MALDI phenomena. The rationale behind and elements of the model are reviewed, including the photophysics, kinetics, and thermodynamics of primary and secondary reaction steps. Experimental results are compared with model predictions to illustrate the foundations of the model, coupling of ablation and ionization, differences between and commonalities of matrices, secondary charge transfer reactions, ionization in both polarities, fluence and concentration dependencies, and suppression and enhancement effects.
紫外激光解吸/电离（MALDI）的物理化学动力学模型已经再现和解释了各种 MALDI 现象。本文回顾了模型的基本原理和要素，包括初级和次级反应步骤的光物理、动力学和热力学。将实验结果与模型预测进行了比较，以说明模型的基础、烧蚀和电离的耦合、基质的差异和共性、次级电荷转移反应、正负极性的电离、光强和浓度依赖性以及抑制和增强效应。
