FOM-Institute AMOLF, Science Park 104, 1098 XG, Amsterdam, The Netherlands.
Maastricht Multimodal Molecular Imaging Institute (M4I), University of Maastricht, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands.
J Am Soc Mass Spectrom. 2017 Nov;28(11):2462-2468. doi: 10.1007/s13361-017-1766-0. Epub 2017 Aug 11.
Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) has been employed to rapidly screen longitudinally sectioned drug user hair samples for cocaine and its metabolites using continuous raster imaging. Optimization of the spatial resolution and raster speed were performed on intact cocaine contaminated hair samples. The optimized settings (100 × 150 μm at 0.24 mm/s) were subsequently used to examine longitudinally sectioned drug user hair samples. The MALDI-MS/MS images showed the distribution of the most abundant cocaine product ion at m/z 182. Using the optimized settings, multiple hair samples obtained from two users were analyzed in approximately 3 h: six times faster than the standard spot-to-spot acquisition method. Quantitation was achieved using longitudinally sectioned control hair samples sprayed with a cocaine dilution series. A multiple reaction monitoring (MRM) experiment was also performed using the 'dynamic pixel' imaging method to screen for cocaine and a range of its metabolites, in order to differentiate between contaminated hairs and drug users. Cocaine, benzoylecgonine, and cocaethylene were detectable, in agreement with analyses carried out using the standard LC-MS/MS method. Graphical Abstract ᅟ.
基质辅助激光解吸/电离-质谱成像（MALDI-MSI）已被用于通过连续光栅成像快速筛选纵向切片的药物使用者头发样本中的可卡因及其代谢物。在完整的可卡因污染头发样本上进行了空间分辨率和光栅速度的优化。随后使用优化的设置（100×150μm，0.24mm/s）检查纵向切片的药物使用者头发样本。MALDI-MS/MS 图像显示了 m/z 182 处最丰富的可卡因产物离子的分布。使用优化的设置，大约 3 小时内分析了来自两个使用者的多个头发样本：比标准点到点采集方法快六倍。使用纵向切片的对照头发样本进行定量分析，这些头发样本喷洒了一系列可卡因稀释液。还使用“动态像素”成像方法进行了多重反应监测（MRM）实验，以筛选可卡因及其一系列代谢物，以便区分污染头发和药物使用者。可卡因、苯甲酰古柯碱和古柯乙二醇均可检测到，与使用标准 LC-MS/MS 方法进行的分析一致。
