Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood , Zhejiang Gongshang University , Hangzhou 310012 , China.
Zhejiang Province Key Lab of Anesthesiology , The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University , Wenzhou 325035 , China.
J Agric Food Chem. 2019 Apr 24;67(16):4679-4688. doi: 10.1021/acs.jafc.9b00751. Epub 2019 Apr 12.
The domestic rainbow trout producers issued a standard with an aquatic association that classified rainbow trout as salmon, which raised the concern of consumers on the fish parasites infection. Herein, an in situ method was developed using "iKnife" and rapid evaporative ionization mass spectrometry based lipidomics for real-time discrimination of salmon and rainbow trout without sample preparation. A total of 12 fatty acids and 37 phospholipid species was identified and imported into statistical analysis for building an in situ and real-time recognition model. The ions with | p(corr)| > 0.5 and | p| > 0.03 were shown to be responsible for allocating samples, and the ions with high correlation values, such as of m/ z 747.50, 771.49, and 863.55, indicated large weights in identification of the salmon and rainbow trout. The results indicated that this technology could be employed as a front-line test method to ensure the authenticity of salmon products.
国内虹鳟鱼生产商与水产协会发布了一项标准，将虹鳟鱼归类为鲑鱼，这引起了消费者对鱼类寄生虫感染的担忧。在此，我们开发了一种使用“iKnife”和基于快速蒸发电离质谱的脂质组学的原位方法，无需样品制备即可实时区分鲑鱼和虹鳟鱼。共鉴定出 12 种脂肪酸和 37 种磷脂，并将其导入统计分析中，以建立原位和实时识别模型。| p(corr)| > 0.5 和 | p| > 0.03 的离子被证明可用于分配样本，而具有高相关值的离子，如 m/ z 747.50、771.49 和 863.55，则表明在识别鲑鱼和虹鳟鱼方面具有较大的权重。结果表明，该技术可作为一线测试方法，以确保鲑鱼产品的真实性。
