Tsunoda Y
FEBS Lett. 1986 Oct 20;207(1):47-52. doi: 10.1016/0014-5793(86)80010-2.
In digitonin-permeabilized parietal cells, myo-inositol 1,4,5-trisphosphate (Ins P3) or Ca2+ ionophore (A23187) increased the cytosolic Ca2+ concentration due to the intracellular Ca2+ release. Addition of ATP decreased the cytosolic Ca2+ concentration due to the rapid Ca2+ re-uptake into the same or similar pool which releases Ca2+ from a non-mitochondrial location (measured by quin2/AM and 45Ca2+). Cytochalasin B failed to increase the cytosolic Ca2+ concentration in response to Ins P3 or A23187 and even failed to decrease the cytosolic Ca2+ concentration in response to ATP. This implies that the ATP-dependent and Ins P3-sensitive Ca2+ pool is linked with the microfilaments of the parietal cell. In intact parietal cells, A23187 increased the amino[14C]pyrine accumulation (an index of acid secretion), that was independent of medium Ca2+. This increase of acid secretion was inhibited by the pretreatment with cytochalasin B. This suggests that medium Ca2+-independent acid secretion (by A23187) is regulated by the microfilaments. Therefore, there is a close relationship between the intracellular Ca2+ metabolism, microfilaments and acid secretion.
在经洋地黄皂苷通透处理的壁细胞中，肌醇1,4,5 - 三磷酸（Ins P3）或钙离子载体（A23187）因细胞内钙释放而增加了胞质钙浓度。添加ATP后，由于钙迅速重新摄取到同一个或类似的从非线粒体位置释放钙的池中（通过喹啉2/AM和45Ca2+测量），胞质钙浓度降低。细胞松弛素B未能响应Ins P3或A23187增加胞质钙浓度，甚至未能响应ATP降低胞质钙浓度。这意味着依赖ATP且对Ins P3敏感的钙池与壁细胞的微丝相关联。在完整的壁细胞中，A23187增加了氨基[14C]吡啉的积累（酸分泌的一个指标），这与培养基中的钙无关。这种酸分泌的增加被细胞松弛素B预处理所抑制。这表明（由A23187引起的）不依赖培养基钙的酸分泌受微丝调节。因此，细胞内钙代谢、微丝和酸分泌之间存在密切关系。
